PMID- 25562583
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20150120
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 31
IP  - 2
DP  - 2015 Jan 20
TI  - Enhanced protein adsorption and facilitated refolding of like-charged protein 
      with highly charged silica nanoparticles fabricated by sequential double 
      modifications.
PG  - 655-8
LID - 10.1021/la5040454 [doi]
AB  - Silica nanoparticles (SNPs) were sequentially modified with poly(ethylenimine) 
      (PEI) and 2-diethylaminoethyl chloride (DEAE) to prepare a series of positively 
      charged SNPs-PEI and SNPs-PEI-DEAE. The sequential double-modification strategy 
      produced a charge density as high as 1740 mumol/g (4524 mumol/mL), which offered a 
      very high adsorption capacity for bovine serum albumin (314 mg/g). Most 
      importantly, the highly charged SNPs-PEI and SNPs-PEI-DEAE could efficiently 
      facilitate the refolding of like-charged protein at extremely low utilization. 
      For instance, in the refolding of 1 mg/mL lysozyme, the refolding yield reached 
      75% with only 3.3 muL/mL SNPs-PEI-DEAE. The bead consumption was reduced by nearly 
      96% as compared to that of the charged microspheres used previously to reach a 
      similar yield. The results proved that the polyelectrolyte-modified SNPs were 
      promising for applications in facilitating like-charged protein refolding, and 
      the research opened up a new way for biotechnology applications of highly charged 
      nanoparticles.
FAU - Liu, Hu
AU  - Liu H
AD  - Department of Biochemical Engineering and Key Laboratory of Systems 
      Bioengineering of the Ministry of Education, School of Chemical Engineering and 
      Technology, Tianjin University , Tianjin 300072, China.
FAU - Dong, Xiaoyan
AU  - Dong X
FAU - Sun, Yan
AU  - Sun Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Cattle
MH  - Nanoparticles/*chemistry
MH  - Polyethyleneimine/chemistry
MH  - Polymers/chemistry
MH  - Protein Refolding
MH  - Proteins/*chemistry
MH  - Silicon Dioxide/*chemistry
EDAT- 2015/01/07 06:00
MHDA- 2015/09/29 06:00
CRDT- 2015/01/07 06:00
PHST- 2015/01/07 06:00 [entrez]
PHST- 2015/01/07 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
AID - 10.1021/la5040454 [doi]
PST - ppublish
SO  - Langmuir. 2015 Jan 20;31(2):655-8. doi: 10.1021/la5040454. Epub 2015 Jan 7.