PMID- 25562743
OWN - NLM
STAT- MEDLINE
DCOM- 20151125
LR  - 20181202
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 68
DP  - 2015 Jun 15
TI  - Simultaneous quantitative detection of multiple tumor markers with a rapid and 
      sensitive multicolor quantum dots based immunochromatographic test strip.
PG  - 156-162
LID - S0956-5663(14)01005-7 [pii]
LID - 10.1016/j.bios.2014.12.051 [doi]
AB  - A novel multicolor quantum dots (QDs) based immunochromatographic test strip 
      (ICTS) was developed for simultaneous quantitative detection of multiple tumor 
      markers, by utilizing alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) 
      as models. The immunosensor could realize simultaneous quantitative detection of 
      tumor markers with only one test line and one control line on the nitrocellulose 
      membrane (NC membrane) due to the introduction of multicolor QDs. In this method, 
      a mixture of mouse anti-AFP McAb and mouse anti-CEA McAb was coated on NC 
      membrane as test line and goat anti-mouse IgG antibody was coated as control 
      line. Anti-AFP McAb-QDs546 conjugates and anti-CEA McAb-QDs620 conjugates were 
      mixed and applied to the conjugate pad. Simultaneous quantitative detection of 
      multiple tumor markers was achieved by detecting the fluorescence intensity of 
      captured QDs labels on test line and control line using a test strip reader. 
      Under the optimum conditions, AFP and CEA could be detected as low as 3 ng/mL and 
      2 ng/mL in 15 min with a sample volume of 80 muL, and no obvious cross-reactivity 
      was observed. The immunosensor was validated with 130 clinical samples and in 
      which it exhibited high sensitivity (93% for AFP and 87% for CEA) and specificity 
      (94% for AFP and 97% for CEA). The immunosensor also demonstrated high recoveries 
      (87.5-113% for AFP and 90-97.3% for CEA) and low relative standard deviations 
      (RSDs) (2.8-6.2% for AFP and 4.9-9.6% for CEA) when testing spiked human serum. 
      This novel multicolor QDs based ICTS provides an easy and rapid, simultaneous 
      quantitative detecting strategy for point-of-care testing of tumor markers.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wang, Chunying
AU  - Wang C
AD  - Schlool of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu 610054, China.
FAU - Hou, Fei
AU  - Hou F
AD  - Schlool of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu 610054, China.
FAU - Ma, Yicai
AU  - Ma Y
AD  - Schlool of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu 610054, China. Electronic address: yicaima@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141223
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Animals
MH  - Antibodies/chemistry
MH  - Biomarkers, Tumor/chemistry/*isolation & purification
MH  - *Biosensing Techniques
MH  - Carcinoembryonic Antigen/chemistry/*isolation & purification
MH  - Chromatography, Affinity
MH  - Humans
MH  - Mice
MH  - Neoplasms/*diagnosis
MH  - Quantum Dots/chemistry
MH  - alpha-Fetoproteins/chemistry/*isolation & purification
OTO - NOTNLM
OT  - Immunochromatographic test strip
OT  - Multicolor quantum dots
OT  - Multiple tumor markers
OT  - One test line
OT  - Simultaneous quantitative detection
EDAT- 2015/01/07 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/01/07 06:00
PHST- 2014/09/27 00:00 [received]
PHST- 2014/12/09 00:00 [revised]
PHST- 2014/12/22 00:00 [accepted]
PHST- 2015/01/07 06:00 [entrez]
PHST- 2015/01/07 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0956-5663(14)01005-7 [pii]
AID - 10.1016/j.bios.2014.12.051 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2015 Jun 15;68:156-162. doi: 10.1016/j.bios.2014.12.051. 
      Epub 2014 Dec 23.