PMID- 25562817
OWN - NLM
STAT- MEDLINE
DCOM- 20151110
LR  - 20171116
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 442
DP  - 2015 Mar 10
TI  - Leukocyte common antigen (CD45) negative follicular lymphoma, a rare 
      immunophenotypic presentation.
PG  - 46-8
LID - S0009-8981(14)00567-1 [pii]
LID - 10.1016/j.cca.2014.12.033 [doi]
AB  - BACKGROUND: CD45 is universally expressed on nucleated hematopoietic cells in 
      healthy individuals with variable intensities. Although down-regulation of CD45 
      expression is commonly seen in immature B-cell neoplasm, loss of CD45 expression 
      is rarely observed in mature B-cell lymphoproliferative disorders. CASE REPORT: A 
      64-y old man was presented with fatigue and night sweat in July 2013. Physical 
      examination revealed right axillary lymphadenopathy without splenohepatomegaly. 
      The lymph node was sent for flow cytometry and histomorphologic evaluation. Flow 
      cytometric analysis revealed the presence of CD45+ and CD45- cell populations. 
      The CD45+ population (42%) comprised mainly unremarkable CD3+ T cells. However, 
      the CD45- population (35%) showed CD19+, CD20+, and CD10+ immunophenotype with 
      monotypic surface Lambda immunoglobulin light chain expression. 
      Immunohistochemical studies performed on tissue section revealed that neoplastic 
      lymphoid nodules were positive for CD20, BCL-6 and BCL-2 with low proliferation 
      index by Ki-67 staining, but again negative for CD45. FISH analysis confirmed the 
      presence of IGH/BCL2 rearrangement. Follicular lymphoma (grade 1) with negative 
      CD45 expression was diagnosed. CONCLUSIONS: Loss of CD45 expression has been 
      reported, but all cases documented were large B cell lymphoma (LBCL). To the best 
      of our knowledge, this is the first case of low grade follicular lymphoma with 
      lack of CD45 expression. We report this case because of not only its rarity but 
      also to raise awareness that loss of CD45 expression can occur in LBCL as well as 
      in low grade B-cell lymphomas.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
FAU - Xu, Dongsheng
AU  - Xu D
AD  - Department of Hematopathology, CBLPath, Inc., Rye Brook, NY, United States.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, China. 
      Electronic address: cuiw@pumch.cn.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20150103
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Immunophenotyping
MH  - Leukocyte Common Antigens/*metabolism
MH  - Lymphoma, Follicular/diagnosis/*immunology/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
OTO - NOTNLM
OT  - CD45
OT  - Flow cytometry
OT  - Follicular lymphoma
EDAT- 2015/01/07 06:00
MHDA- 2015/11/11 06:00
CRDT- 2015/01/07 06:00
PHST- 2014/11/15 00:00 [received]
PHST- 2014/12/26 00:00 [revised]
PHST- 2014/12/29 00:00 [accepted]
PHST- 2015/01/07 06:00 [entrez]
PHST- 2015/01/07 06:00 [pubmed]
PHST- 2015/11/11 06:00 [medline]
AID - S0009-8981(14)00567-1 [pii]
AID - 10.1016/j.cca.2014.12.033 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2015 Mar 10;442:46-8. doi: 10.1016/j.cca.2014.12.033. Epub 2015 
      Jan 3.