PMID- 25563385
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20231213
IS  - 2047-783X (Electronic)
IS  - 0949-2321 (Print)
IS  - 0949-2321 (Linking)
VI  - 20
IP  - 1
DP  - 2015 Jan 7
TI  - An immunohistochemical study of CD83- and CD1a-positive dendritic cells in the 
      decidua of women with recurrent spontaneous abortion.
PG  - 2
LID - 10.1186/s40001-014-0076-2 [doi]
LID - 2
AB  - BACKGROUND: There are more and more women with recurrent spontaneous abortion 
      (RSA). The mechanism of RSA is still unclear. Immunological factors have been 
      postulated to play a role in the etiology of RSA. Dendritic cells (DCs) are the 
      most potent antigen-presenting cells in the immune system, and the decidual DCs 
      may take part in the occurrence of RSA. The difference in maturity status of 
      decidual DCs among women with RSA and women with normal pregnancies is worthy of 
      studying for its application to prevention and therapy. METHODS: The EnVision 
      two-step immunohistochemical staining technique was used to detect the expression 
      of CD83 and CD1a in the decidua of women with RSA (30 cases) and normal 
      pregnancies (30 cases). The maturity status, distribution and quantity of DCs in 
      the two groups were observed. Observation of the staining and cell counting were 
      done using microscope within 30 randomly selected high-power fields (HPF, 40 x 
      10). All data analyses were conducted with SPSS 17.0 and the statistical 
      significance was set at P <0.05. RESULTS: The decidua from the two groups 
      contained DCs that stained with the anti-CD83 and anti-CD1a antibody. Most of the 
      decidual CD83(+)DCs from two groups were located in the stroma. There were more 
      CD83(+)DCs clustered with other DCs in the stroma from women with RSA than normal 
      pregnancies. Most of the CD1a(+)DCs in the decidua from the two groups are 
      located close to maternal glandular epithelium. No difference in the location of 
      CD1a(+)DCs was found in the decidua between two groups. The number of decidual 
      CD83(+)DCs was statistically significantly higher in RSA women than in normal 
      early pregnant women (14.20 +/- 13.34/30 HPF versus 4.77 +/- 2.64/30 HPF; t = 3.800, 
      P = 0.001). The number of CD1a(+)DCs in the decidua was statistically 
      significantly lower in RSA women compared with normal early pregnant women (3.97 
      +/- 3.75/30 HPF versus 7.60 +/- 6.08/30 HPF; t = 2.786, P = 0.008). CONCLUSIONS: 
      These findings suggest that the increase in the number of mature DCs and the 
      decrease in the quantity of immature DCs in the decidua may be related to RSA. 
      The maturation of decidual DCs may play an important role in the pathogenesis of 
      RSA.
FAU - Qian, Zhi-Da
AU  - Qian ZD
AD  - Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, 
      Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang Province, 310006, People's 
      Republic of China. qianada@163.com.
FAU - Huang, Li-Li
AU  - Huang LL
AD  - Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, 
      Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang Province, 310006, People's 
      Republic of China. fbhuanglili@163.com.
FAU - Zhu, Xiao-Ming
AU  - Zhu XM
AD  - Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, 
      Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang Province, 310006, People's 
      Republic of China. fbzhuxiaoming@163.com.
AD  - Key Laboratory of Reproductive Genetics (Zhejiang University), Ministry of 
      Education, Hangzhou, People's Republic of China. fbzhuxiaoming@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - England
TA  - Eur J Med Res
JT  - European journal of medical research
JID - 9517857
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Abortion, Habitual/*immunology
MH  - Adult
MH  - Antigens, CD/genetics/*metabolism
MH  - Antigens, CD1/genetics/*metabolism
MH  - Case-Control Studies
MH  - Cell Differentiation
MH  - Decidua/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulins/genetics/*metabolism
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - CD83 Antigen
PMC - PMC4301856
EDAT- 2015/01/08 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/01/07
CRDT- 2015/01/08 06:00
PHST- 2014/01/20 00:00 [received]
PHST- 2014/12/08 00:00 [accepted]
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/01/07 00:00 [pmc-release]
AID - s40001-014-0076-2 [pii]
AID - 76 [pii]
AID - 10.1186/s40001-014-0076-2 [doi]
PST - epublish
SO  - Eur J Med Res. 2015 Jan 7;20(1):2. doi: 10.1186/s40001-014-0076-2.