PMID- 25563793
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20150209
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 163
IP  - 3-4
DP  - 2015 Feb 15
TI  - The role of TREM-2 in internalization and intracellular survival of Brucella 
      abortus in murine macrophages.
PG  - 194-201
LID - S0165-2427(14)00309-2 [pii]
LID - 10.1016/j.vetimm.2014.12.007 [doi]
AB  - Triggering receptor expressed on myeloid cells-2 (TREM-2) is a cell surface 
      receptor primarily expressed on macrophages and dendritic cells. TREM-2 functions 
      as a phagocytic receptor for bacteria as well as an inhibitor of Toll like 
      receptors (TLR) induced inflammatory cytokines. However, the role of TREM-2 in 
      Brucella intracellular growth remains unknown. To investigate whether TREM-2 is 
      involved in Brucella intracellular survival, we chose bone marrow derived 
      macrophages (BMDMs), in which TREM-2 is stably expressed, as cell model. Colony 
      formation Units (CFUs) assay suggests that TREM-2 is involved in the 
      internalization of Brucella abortus (B. abortus) by macrophages, while silencing 
      of TREM-2 decreases intracellular survival of B. abortus. To further study the 
      underlying mechanisms of TREM-2-mediated bacterial intracellular survival, we 
      examined the activation of B. abortus-infected macrophages through determining 
      the kinetics of activation of the three MAPKs, including ERK, JNK and p38, and 
      measuring TNFalpha production in response to lipopolysaccharide (LPS) of Brucella 
      (BrLPS) or B. abortus stimulation. Our data show that TREM-2 deficiency promotes 
      activation of Brucella-infected macrophages. Moreover, our data also demonstrate 
      that macrophage activation promotes killing of Brucella by enhancing nitric 
      oxygen (NO), but not reactive oxygen species (ROS) production, macrophage 
      apoptosis or cellular death. Taken together, these findings provide a novel 
      interpretation of Brucella intracellular growth through inhibition of NO 
      production produced by TREM-2-mediated activated macrophages.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wei, Pan
AU  - Wei P
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Lu, Qiang
AU  - Lu Q
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Cui, Guimei
AU  - Cui G
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Guan, Zhenhong
AU  - Guan Z
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Yang, Li
AU  - Yang L
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Sun, Changjiang
AU  - Sun C
AD  - College of Veterinary Medicine, Jilin University, Changchun, People's Republic of 
      China.
FAU - Sun, Wanchun
AU  - Sun W
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
FAU - Peng, Qisheng
AU  - Peng Q
AD  - Key Laboratory for Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, Jilin University, Changchun 130062, People's Republic of China. 
      Electronic address: Qishengpeng@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141223
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Trem2 protein, mouse)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Brucella abortus/*physiology
MH  - Cytokines/genetics/metabolism
MH  - Gene Expression Regulation/*physiology
MH  - Macrophage Activation/physiology
MH  - Macrophages/*metabolism/*microbiology
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Reactive Oxygen Species
MH  - Receptors, Immunologic/genetics/*metabolism
OTO - NOTNLM
OT  - Brucella abortus
OT  - Macrophage
OT  - NO
OT  - TREM-2
EDAT- 2015/01/08 06:00
MHDA- 2016/02/11 06:00
CRDT- 2015/01/08 06:00
PHST- 2014/08/23 00:00 [received]
PHST- 2014/12/15 00:00 [revised]
PHST- 2014/12/16 00:00 [accepted]
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
AID - S0165-2427(14)00309-2 [pii]
AID - 10.1016/j.vetimm.2014.12.007 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2015 Feb 15;163(3-4):194-201. doi: 
      10.1016/j.vetimm.2014.12.007. Epub 2014 Dec 23.