PMID- 25565312
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20150530
IS  - 1523-1755 (Electronic)
IS  - 0085-2538 (Linking)
VI  - 87
IP  - 6
DP  - 2015 Jun
TI  - Restricted specificity of peripheral alloreactive memory B cells in 
      HLA-sensitized patients awaiting a kidney transplant.
PG  - 1230-40
LID - 10.1038/ki.2014.390 [doi]
AB  - The contribution of memory B cells in alloreactive humoral responses remains 
      poorly understood. Here we tested the presence of circulating alloreactive memory 
      B cells in 69 patients with end-stage renal disease under renal replacement 
      therapy, using an in vitro memory B cell-stimulation assay combined with 
      identification of IgG human leukocyte antigen (HLA) antibodies in culture 
      supernatant. HLA antibody-producing memory B cells were evidenced only in 
      patients carrying serum HLA antibodies following multiple classical 
      HLA-immunizing events. In patients with a previous renal allograft, alloreactive 
      memory B cells could be detected ranging from 6 to 32 years (mean 13.2 years) 
      after transplantation. HLA antibodies produced by memory B cells were also 
      detected in the corresponding sera and showed a restricted reactivity, targeting 
      only a few epitopes shared by several HLA antigens. In contrast, serum HLA 
      antibodies, not associated with the detection of specific memory B cells, showed 
      a broader pattern of specificities. Thus, expansion and survival of alloreactive 
      memory B cells is alloantigen driven, and their frequency is related to the 
      'strength' of HLA immunization.
FAU - Snanoudj, Renaud
AU  - Snanoudj R
AD  - 1] Institut Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Hopital Necker, 
      Paris, France [2] Universite Paris Descartes, Sorbonne Paris Cite, Paris, France 
      [3] Service de Transplantation Renale Adulte, Hopital Necker, Assistance 
      Publique-Hopitaux de Paris, Paris, France.
FAU - Claas, Frans H J
AU  - Claas FH
AD  - Department of Immunohaematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, The Netherlands.
FAU - Heidt, Sebastiaan
AU  - Heidt S
AD  - Department of Immunohaematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, The Netherlands.
FAU - Legendre, Christophe
AU  - Legendre C
AD  - 1] Universite Paris Descartes, Sorbonne Paris Cite, Paris, France [2] Service de 
      Transplantation Renale Adulte, Hopital Necker, Assistance Publique-Hopitaux de 
      Paris, Paris, France.
FAU - Chatenoud, Lucienne
AU  - Chatenoud L
AD  - 1] Institut Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Hopital Necker, 
      Paris, France [2] Universite Paris Descartes, Sorbonne Paris Cite, Paris, France.
FAU - Candon, Sophie
AU  - Candon S
AD  - 1] Institut Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Hopital Necker, 
      Paris, France [2] Universite Paris Descartes, Sorbonne Paris Cite, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (HLA Antigens)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adult
MH  - B-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Immunity, Humoral
MH  - Immunoglobulin G/analysis/*blood
MH  - *Immunologic Memory
MH  - Kidney Failure, Chronic/*immunology/surgery
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Time Factors
EDAT- 2015/01/08 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/01/08 06:00
PHST- 2014/07/04 00:00 [received]
PHST- 2014/10/01 00:00 [revised]
PHST- 2014/10/09 00:00 [accepted]
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0085-2538(15)30139-3 [pii]
AID - 10.1038/ki.2014.390 [doi]
PST - ppublish
SO  - Kidney Int. 2015 Jun;87(6):1230-40. doi: 10.1038/ki.2014.390. Epub 2015 Jan 7.