PMID- 25566087
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150108
LR  - 20220311
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 5
DP  - 2014
TI  - Satellite cell activity is differentially affected by contraction mode in human 
      muscle following a work-matched bout of exercise.
PG  - 485
LID - 10.3389/fphys.2014.00485 [doi]
LID - 485
AB  - Optimal repair and adaptation of skeletal muscle is facilitated by resident stem 
      cells (satellite cells). To understand how different exercise modes influence 
      satellite cell dynamics, we measured satellite cell activity in conjunction with 
      markers of muscle damage and inflammation in human skeletal muscle following a 
      single work- and intensity-matched bout of eccentric (ECC) or concentric 
      contractions (CON). Participants completed a single bout of ECC (n = 7) or CON (n 
      = 7) of the knee extensors. A muscle biopsy was obtained before and 24 h after 
      exercise. Functional measures and immunohistochemical analyses were used to 
      determine the extent of muscle damage and indices of satellite cell activity. 
      Cytokine concentrations were measured using a multiplexed magnetic bead assay. 
      Isokinetic peak torque decreased following ECC (p < 0.05) but not CON. Greater 
      histological staining of the damage marker Xin was observed in muscle samples of 
      ECC vs. CON. Tenasin C immunoreactivity increased 15 fold (p < 0.01) following 
      ECC and was unchanged following CON. The inflammatory cytokines interferon 
      gamma-induced protein 10 (IP-10) and monocyte chemotactic protein 1 (MCP-1) 
      increased pre- to post-ECC (4.26 +/- 1.4 vs. 10.49 +/- 5.8 pg/ml, and 3.06 +/- 0.7 vs. 
      6.25 +/- 4.6 pg/ml, respectively; p < 0.05). There was no change in any cytokine 
      post-CON. Satellite cell content increased 27% pre- to post-ECC (0.10 +/- 0.031 vs. 
      0.127 +/- 0.041, respectively; p < 0.05). There was no change in satellite cell 
      number in CON (0.099 +/- 0.027 vs. 0.102 +/- 0.029, respectively). There was no fiber 
      type-specific satellite cell response following either exercise mode. ECC but not 
      CON resulted in an increase in MyoD positive nuclei per myofiber pre- to 
      post-exercise (p < 0.05), but there was no change in MyoD DNA binding activity in 
      either condition. In conclusion, ECC but not CON results in functional and 
      histological evidence of muscle damage that is accompanied by increased satellite 
      cell activity 24 h post-exercise.
FAU - Hyldahl, Robert D
AU  - Hyldahl RD
AD  - Department of Exercise Sciences, Brigham Young University Provo, UT, USA.
FAU - Olson, Ty
AU  - Olson T
AD  - Department of Exercise Sciences, Brigham Young University Provo, UT, USA.
FAU - Welling, Tyson
AU  - Welling T
AD  - Department of Exercise Sciences, Brigham Young University Provo, UT, USA.
FAU - Groscost, Logan
AU  - Groscost L
AD  - Department of Exercise Sciences, Brigham Young University Provo, UT, USA.
FAU - Parcell, Allen C
AU  - Parcell AC
AD  - Department of Exercise Sciences, Brigham Young University Provo, UT, USA.
LA  - eng
PT  - Journal Article
DEP - 20141211
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC4263080
OTO - NOTNLM
OT  - MyoD
OT  - Pax7
OT  - concentric
OT  - eccentric
OT  - resistance exercise
OT  - stem cell
EDAT- 2015/01/08 06:00
MHDA- 2015/01/08 06:01
PMCR- 2014/12/11
CRDT- 2015/01/08 06:00
PHST- 2014/09/19 00:00 [received]
PHST- 2014/11/25 00:00 [accepted]
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2015/01/08 06:01 [medline]
PHST- 2014/12/11 00:00 [pmc-release]
AID - 10.3389/fphys.2014.00485 [doi]
PST - epublish
SO  - Front Physiol. 2014 Dec 11;5:485. doi: 10.3389/fphys.2014.00485. eCollection 
      2014.