PMID- 25566327
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150108
LR  - 20200929
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 5
DP  - 2014
TI  - Developmental regulation of ecdysone receptor (EcR) and EcR-controlled gene 
      expression during pharate-adult development of honeybees (Apis mellifera).
PG  - 445
LID - 10.3389/fgene.2014.00445 [doi]
LID - 445
AB  - Major developmental transitions in multicellular organisms are driven by steroid 
      hormones. In insects, these, together with juvenile hormone (JH), control 
      development, metamorphosis, reproduction and aging, and are also suggested to 
      play an important role in caste differentiation of social insects. Here, we aimed 
      to determine how EcR transcription and ecdysteroid titers are related during 
      honeybee postembryonic development and what may actually be the role of EcR in 
      caste development of this social insect. In addition, we expected that 
      knocking-down EcR gene expression would give us information on the participation 
      of the respective protein in regulating downstream targets of EcR. We found that 
      in Apis mellifera females, EcR-A is the predominantly expressed variant in 
      postembryonic development, while EcR-B transcript levels are higher in embryos, 
      indicating an early developmental switch in EcR function. During larval and pupal 
      stages, EcR-B expression levels are very low, while EcR-A transcripts are more 
      variable and abundant in workers compared to queens. Strikingly, these transcript 
      levels are opposite to the ecdysteroid titer profile. 20-hydroxyecdysone (20E) 
      application experiments revealed that low 20E levels induce EcR expression during 
      development, whereas high ecdysteroid titers seem to be repressive. By means of 
      RNAi-mediated knockdown (KD) of both EcR transcript variants we detected the 
      differential expression of 234 poly-A(+) transcripts encoding genes such as CYPs, 
      MRJPs and certain hormone response genes (Kr-h1 and ftz-f1). EcR-KD also promoted 
      the differential expression of 70 miRNAs, including highly conserved ones (e.g., 
      miR-133 and miR-375), as well honeybee-specific ones (e.g., miR-3745 and 
      miR-3761). Our results put in evidence a broad spectrum of EcR-controlled gene 
      expression during postembryonic development of honeybees, revealing new facets of 
      EcR biology in this social insect.
FAU - Mello, Tathyana R P
AU  - Mello TR
AD  - Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo Sao Paulo, Brazil.
FAU - Aleixo, Aline C
AU  - Aleixo AC
AD  - Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo Sao Paulo, Brazil.
FAU - Pinheiro, Daniel G
AU  - Pinheiro DG
AD  - Faculdade de Ciencias Agrarias e Veterinarias, Universidade Estadual Paulista Sao 
      Paulo, Brazil.
FAU - Nunes, Francis M F
AU  - Nunes FM
AD  - Departamento de Genetica e Evolucao, Centro de Ciencias Biologicas e da Saude, 
      Universidade Federal de Sao Carlos Sao Carlos, Brazil.
FAU - Bitondi, Marcia M G
AU  - Bitondi MM
AD  - Departamento de Biologia, Faculdade de Filosofia, Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo Sao Paulo, Brazil.
FAU - Hartfelder, Klaus
AU  - Hartfelder K
AD  - Departamento de Biologia Celular, Molecular e de Bioagentes Patogenicos, 
      Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo Sao Paulo, 
      Brazil.
FAU - Barchuk, Angel R
AU  - Barchuk AR
AD  - Laboratorio de Biologia Animal Integrativa, Departamento de Biologia Celular, 
      Tecidual e do Desenvolvimento, Instituto de Ciencias Biomedicas, Universidade 
      Federal de Alfenas Alfenas, Brazil.
FAU - Simoes, Zila L P
AU  - Simoes ZL
AD  - Departamento de Biologia, Faculdade de Filosofia, Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20141222
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC4273664
OTO - NOTNLM
OT  - 20E
OT  - JH
OT  - RNAi
OT  - adult development
OT  - ecdysteroid
OT  - honey bee
OT  - juvenile hormone
OT  - miRNA
EDAT- 2015/01/08 06:00
MHDA- 2015/01/08 06:01
PMCR- 2014/12/22
CRDT- 2015/01/08 06:00
PHST- 2014/09/28 00:00 [received]
PHST- 2014/12/04 00:00 [accepted]
PHST- 2015/01/08 06:00 [entrez]
PHST- 2015/01/08 06:00 [pubmed]
PHST- 2015/01/08 06:01 [medline]
PHST- 2014/12/22 00:00 [pmc-release]
AID - 10.3389/fgene.2014.00445 [doi]
PST - epublish
SO  - Front Genet. 2014 Dec 22;5:445. doi: 10.3389/fgene.2014.00445. eCollection 2014.