PMID- 25567591
OWN - NLM
STAT- MEDLINE
DCOM- 20150610
LR  - 20181202
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 43
IP  - 10
DP  - 2014 Oct
TI  - [Correlation of chromosome 1p and 19q status and expression of R132H mutant IDH1 
      protein in oligodendroglial tumors].
PG  - 663-7
AB  - OBJECTIVE: To correlate the presence of chromosome 1p/19q deletion with the 
      expression of R132H mutant IDH1 status in oligodendroglial tumors, and to explore 
      molecular markers for predicting chemosensitivity of oligodendroglial tumors. 
      METHODS: The study included 75 oligodendroglial tumors (38 oligodendrogliomas and 
      37 oligoastrocytomas). Immunohistochemistry was used to detect the expression of 
      R132H mutant IDH1 protein, and fluorescence in situ hybridization (FISH) was 
      employed to detect 1p/19q deletion. RESULTS: Deletion of chromosome 1p and/or 19q 
      was detected in 37 cases (37/75, 49.3%), among which co-deletion of 1p and 19q 
      was seen in 34 cases (closely correlated, P < 0.01). Oligodendrogliomas WHOIIhad 
      a slightly higher deletion rate than oligodendrogliomas WHO III, although without 
      statistical significance. Oligodendrogliomas WHO IIand WHO III had a 
      significantly higher deletion rate of chromosome 1p/19q than oligoastrocytomas 
      WHO II and WHO III (P < 0.05). While combined loss of 1p/19q was always detected 
      in oligodendrogliomas when FISH was positive, isolated 1p or 19q deletion was 
      only found in oligoastrocytomas. The expression of R132H mutant IDH1 was detected 
      in 51 of 75 cases (68.0%), in which oligodendrogliomas had a higher positive rate 
      than oligoastrocytomas. Statistical analysis demonstrated a significant 
      correlation between the expression of R132H mutant IDH1 protein and the presence 
      of combined 1p/19q deletion in oligodendrogliomas (P < 0.05). CONCLUSIONS: A 
      significant correlation was observed between the expression of R132H mutant 
      protein and 1p/19q LOH.Expression of 132H mutant IDH1 protein is the potential 
      biomarker for predicating the presence of 1p/19q deletion in oligodendrogliomas.
FAU - Yao, Kun
AU  - Yao K
AD  - Department of Pathology, Beijing Sanbo Brain Hospital, Capital Medical 
      University, Beijing 100093, China.
FAU - Duan, Zejun
AU  - Duan Z
AD  - Department of Pathology, Beijing Sanbo Brain Hospital, Capital Medical 
      University, Beijing 100093, China.
FAU - Hu, Zeliang
AU  - Hu Z
AD  - Department of Pathology, Beijing Sanbo Brain Hospital, Capital Medical 
      University, Beijing 100093, China.
FAU - Bian, Yu
AU  - Bian Y
AD  - Department of Pathology, Beijing Sanbo Brain Hospital, Capital Medical 
      University, Beijing 100093, China.
FAU - Qi, Xueling
AU  - Qi X
AD  - Department of Pathology, Beijing Sanbo Brain Hospital, Capital Medical 
      University, Beijing 100093, China. E-mail: xszqxl@sohu.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Mutant Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - EC 1.1.1.41 (Isocitrate Dehydrogenase)
RN  - EC 1.1.1.42. (IDH1 protein, human)
RN  - Chromosome 1, monosomy 1p
SB  - IM
MH  - Aged
MH  - Brain Neoplasms/*genetics/metabolism
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, 19-20/*genetics
MH  - Chromosomes, Human, Pair 1
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Isocitrate Dehydrogenase/*genetics/metabolism
MH  - Middle Aged
MH  - Mutant Proteins/*metabolism
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Oligodendroglioma/*genetics/metabolism
EDAT- 2015/01/09 06:00
MHDA- 2015/06/11 06:00
CRDT- 2015/01/09 06:00
PHST- 2015/01/09 06:00 [entrez]
PHST- 2015/01/09 06:00 [pubmed]
PHST- 2015/06/11 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2014 Oct;43(10):663-7.