PMID- 25568127
OWN - NLM
STAT- MEDLINE
DCOM- 20150320
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 35
IP  - 1
DP  - 2015 Jan 7
TI  - Chromatin landscape defined by repressive histone methylation during 
      oligodendrocyte differentiation.
PG  - 352-65
LID - 10.1523/JNEUROSCI.2606-14.2015 [doi]
AB  - In many cell types, differentiation requires an interplay between extrinsic 
      signals and transcriptional changes mediated by repressive and activating histone 
      modifications. Oligodendrocyte progenitors (OPCs) are electrically responsive 
      cells receiving synaptic input. The differentiation of these cells into 
      myelinating oligodendrocytes is characterized by temporal waves of gene 
      repression followed by activation of myelin genes and progressive decline of 
      electrical responsiveness. In this study, we used chromatin isolated from rat 
      OPCs and immature oligodendrocytes, to characterize the genome-wide distribution 
      of the repressive histone marks, H3K9me3 and H3K27me3, during differentiation. 
      Although both marks were present at the OPC stage, only H3K9me3 marks (but not 
      H3K27me3) were found to be increased during differentiation, at genes related to 
      neuronal lineage and regulation of membrane excitability. Consistent with these 
      findings, the levels and activity of H3K9 methyltransferases (H3K9 HMT), but not 
      H3K27 HMT, increased more prominently upon exposure to oligodendrocyte 
      differentiating stimuli and were detected in stage-specific repressive protein 
      complexes containing the transcription factors SOX10 or YY1. Silencing H3K9 HMT, 
      but not H3K27 HMT, impaired oligodendrocyte differentiation and functionally 
      altered the response of oligodendrocytes to electrical stimulation. Together, 
      these results identify repressive H3K9 methylation as critical for gene 
      repression during oligodendrocyte differentiation.
CI  - Copyright (c) 2015 the authors 0270-6474/15/350352-14$15.00/0.
FAU - Liu, Jia
AU  - Liu J
AUID- ORCID: 0000-0002-6457-2935
AD  - Department of Neuroscience.
FAU - Magri, Laura
AU  - Magri L
AD  - Department of Neuroscience.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Bioinformatics Laboratory, Department of Medicine, and.
FAU - Marsh, Nidaa O
AU  - Marsh NO
AUID- ORCID: 0000-0003-2420-7295
AD  - Department of Neuroscience.
FAU - Albrecht, Stefanie
AU  - Albrecht S
AD  - Institute of Neuropathology, University Hospital Munster, D-48149 Munster, 
      Germany.
FAU - Huynh, Jimmy L
AU  - Huynh JL
AD  - Department of Neuroscience, Department of Genetics and Genomic Sciences, Icahn 
      School of Medicine at Mount Sinai, New York, New York 10029, and.
FAU - Kaur, Jasbir
AU  - Kaur J
AUID- ORCID: 0000-0001-6842-2240
AD  - Department of Neuroscience.
FAU - Kuhlmann, Tanja
AU  - Kuhlmann T
AD  - Institute of Neuropathology, University Hospital Munster, D-48149 Munster, 
      Germany.
FAU - Zhang, Weijia
AU  - Zhang W
AD  - Bioinformatics Laboratory, Department of Medicine, and.
FAU - Slesinger, Paul A
AU  - Slesinger PA
AD  - Department of Neuroscience.
FAU - Casaccia, Patrizia
AU  - Casaccia P
AUID- ORCID: 0000-0002-4785-9264
AD  - Department of Neuroscience, Department of Genetics and Genomic Sciences, Icahn 
      School of Medicine at Mount Sinai, New York, New York 10029, and 
      patrizia.casaccia@mssm.edu.
LA  - eng
GR  - R01 NS052738/NS/NINDS NIH HHS/United States
GR  - R37 NS042925/NS/NINDS NIH HHS/United States
GR  - 2R37NS042925-10/NS/NINDS NIH HHS/United States
GR  - R01NS52738/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Chromatin)
RN  - 0 (Histones)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Chromatin/*metabolism
MH  - Chromatin Immunoprecipitation/methods
MH  - Female
MH  - Histones/*metabolism
MH  - Male
MH  - Methylation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neurogenesis/*physiology
MH  - Oligodendroglia/*metabolism
MH  - Organ Culture Techniques
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4287153
OTO - NOTNLM
OT  - ChIP-seq
OT  - epigenetics
OT  - myelin
OT  - potassium channel
EDAT- 2015/01/09 06:00
MHDA- 2015/03/21 06:00
PMCR- 2015/07/07
CRDT- 2015/01/09 06:00
PHST- 2015/01/09 06:00 [entrez]
PHST- 2015/01/09 06:00 [pubmed]
PHST- 2015/03/21 06:00 [medline]
PHST- 2015/07/07 00:00 [pmc-release]
AID - 35/1/352 [pii]
AID - 2606-14 [pii]
AID - 10.1523/JNEUROSCI.2606-14.2015 [doi]
PST - ppublish
SO  - J Neurosci. 2015 Jan 7;35(1):352-65. doi: 10.1523/JNEUROSCI.2606-14.2015.