PMID- 2556814
OWN - NLM
STAT- MEDLINE
DCOM- 19900125
LR  - 20141120
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 62
IP  - 3
DP  - 1989 Nov 24
TI  - The effect of calcium chloride on anti-Xa activity of heparin and its molecular 
      weight fractions.
PG  - 950-4
AB  - The anti-Xa activities of unfractionated heparin (UFH) and nine low molecular 
      weight heparins (LMWH) have been measured in the presence and absence of 3 mM 
      CaCl2, using bovine Factor Xa, purified human ATIII and an amidolytic assay. The 
      addition of CaCl2 increased the activity of UFH by 93%, but the effect on LMWH 
      was less, ranging from -20% to +55%. Studies of gel filtration fractions of UFH 
      showed marked Mr dependence of the CaCl2 effect in the range 4,000-12,000. The 
      differences among the various LMW heparins with respect to the effect of CaCl2 
      were closely correlated with the amount of polysaccharide above an Mr of 6,500. 
      Kinetic studies confirmed the potentiation of the activity of UFH with bovine Xa 
      and showed an even more marked effect using human Xa.
FAU - Barrowcliffe, T W
AU  - Barrowcliffe TW
AD  - National Institute for Biological Standards and Control, Potters Bar, Herts., 
      U.K.
FAU - Le Shirley, Y
AU  - Le Shirley Y
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Factor Xa Inhibitors)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9000-94-6 (Antithrombin III)
RN  - 9005-49-6 (Heparin)
RN  - M4I0D6VV5M (Calcium Chloride)
SB  - IM
MH  - Antithrombin III/pharmacology
MH  - Calcium Chloride/*pharmacology
MH  - *Factor Xa Inhibitors
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - Humans
MH  - Kinetics
EDAT- 1989/11/24 00:00
MHDA- 1989/11/24 00:01
CRDT- 1989/11/24 00:00
PHST- 1989/11/24 00:00 [pubmed]
PHST- 1989/11/24 00:01 [medline]
PHST- 1989/11/24 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1989 Nov 24;62(3):950-4.