PMID- 25571907
OWN - NLM
STAT- MEDLINE
DCOM- 20160927
LR  - 20151009
IS  - 1863-2378 (Electronic)
IS  - 1863-1959 (Linking)
VI  - 62
IP  - 7
DP  - 2015 Nov
TI  - Hybrids of Shigatoxigenic and Enterotoxigenic Escherichia coli (STEC/ETEC) Among 
      Human and Animal Isolates in Finland.
PG  - 518-24
LID - 10.1111/zph.12177 [doi]
AB  - Diarrhoeagenic Escherichia coli (DEC) cause serious foodborne infections in 
      humans. Total of 450 Shigatoxigenic E. coli (STEC) strains isolated from humans, 
      animals and environment in Finland were examined by multiplex PCR targeting the 
      virulence genes of various DEC pathogroups simultaneously. One per cent (3/291) 
      of the human STEC and 14% (22/159) of the animal and environmental STEC had genes 
      typically present in enterotoxigenic E. coli (ETEC). The strains possessed genes 
      encoding both Shiga toxin 1 and/or 2 (stx1 and/or stx2 ) and ETEC-specific 
      heat-stable (ST) enterotoxin Ia (estIa). The identified stx subtypes were stx1a, 
      stx1c, stx2a, stx2d and stx2g. The three human STEC/ETEC strains were isolated 
      from the patients with haemolytic uraemic syndrome and diarrhoea and from an 
      asymptomatic carrier. The animal STEC/ETEC strains were isolated from cattle and 
      moose. The human and animal STEC/ETEC strains belonged to 11 serotypes, of which 
      O2:H27, O15:H16, O101:H-, O128:H8 and O141:H8 have previously been described to 
      be associated with human disease. Identification of multiple virulence genes 
      offers further information for assessing the virulence potential of STEC and 
      other DEC. The emergence of novel hybrid pathogens should be taken into account 
      in the patient care and epidemiological surveillance.
CI  - (c) 2015 Blackwell Verlag GmbH.
FAU - Nyholm, O
AU  - Nyholm O
AD  - Bacteriology Unit, National Institute for Health and Welfare (THL), Helsinki, 
      Finland.
FAU - Heinikainen, S
AU  - Heinikainen S
AD  - Veterinary Bacteriology Research Unit, Finnish Food Safety Authority Evira, 
      Kuopio, Finland.
FAU - Pelkonen, S
AU  - Pelkonen S
AD  - Veterinary Bacteriology Research Unit, Finnish Food Safety Authority Evira, 
      Kuopio, Finland.
FAU - Hallanvuo, S
AU  - Hallanvuo S
AD  - Food and Feed Microbiology Research Unit, Finnish Food Safety Authority Evira, 
      Helsinki, Finland.
FAU - Haukka, K
AU  - Haukka K
AD  - Bacteriology Unit, National Institute for Health and Welfare (THL), Helsinki, 
      Finland.
AD  - Department of Food and Environmental Sciences, University of Helsinki, Helsinki, 
      Finland.
FAU - Siitonen, A
AU  - Siitonen A
AD  - Bacteriology Unit, National Institute for Health and Welfare (THL), Helsinki, 
      Finland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150109
PL  - Germany
TA  - Zoonoses Public Health
JT  - Zoonoses and public health
JID - 101300786
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Carrier State/microbiology
MH  - Cattle
MH  - Cattle Diseases/*microbiology
MH  - Deer/*microbiology
MH  - Diarrhea/microbiology
MH  - Enterotoxigenic Escherichia coli/*genetics/pathogenicity
MH  - Escherichia coli Infections/*genetics/microbiology
MH  - Escherichia coli O157/genetics
MH  - Finland
MH  - Hemolytic-Uremic Syndrome/microbiology
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Serotyping
MH  - Shiga-Toxigenic Escherichia coli/*genetics/pathogenicity
MH  - Virulence Factors/*genetics
OTO - NOTNLM
OT  - Shigatoxigenic Escherichia coli
OT  - emerging pathogenic E. coli
OT  - enterotoxigenic E. coli
OT  - hybrid pathogroup
OT  - multiplex PCR
EDAT- 2015/01/13 06:00
MHDA- 2016/09/28 06:00
CRDT- 2015/01/10 06:00
PHST- 2014/08/22 00:00 [received]
PHST- 2015/01/10 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2016/09/28 06:00 [medline]
AID - 10.1111/zph.12177 [doi]
PST - ppublish
SO  - Zoonoses Public Health. 2015 Nov;62(7):518-24. doi: 10.1111/zph.12177. Epub 2015 
      Jan 9.