PMID- 2557390
OWN - NLM
STAT- MEDLINE
DCOM- 19900130
LR  - 20131121
IS  - 0732-6580 (Print)
IS  - 0732-6580 (Linking)
VI  - 8
IP  - 6
DP  - 1989 Dec
TI  - Comparative effects of F1 and P1 fractions obtained from a Klebsiella pneumoniae 
      glycoproteic extract (RU 41740) on polymorphonuclear leukocytes.
PG  - 656-64
AB  - RU 41740 is a glycoprotein extract from Klebsiella pneumoniae described as a 
      macromolecular aggregation of a lipopolysaccharide (LPS)-associated protein (F1 
      fraction) and a glycoproteic complex (P1 fraction). The human polymorphonuclear 
      (PMN) response was studied after incubation of the cells in the presence of RU 
      41740, F1 and P1 fractions, or F1-P1 complex. Oxidative metabolism was assessed 
      by chemiluminescence, O2 consumption, O2- generation, and degranulation by 
      beta-glucuronidase release. Results were compared to data obtained with a 
      homologous LPS. RU 41740, F1 fraction, and F1-P1 complex increased the 
      respiratory burst of PMNs stimulated by opsonized zymosan (OZ). 
      N-formylmethionylleucylphenylalanine (fMLP), phorbol myristate acetate, or the 
      calcium ionophore A23187. The beta-glucuronidase release was stimulated by the 
      same compounds when OZ or fMLP were used as stimuli. These effects were 
      dose-dependent. In contrast, P1 fraction was inactive. Addition of polymyxin B 
      resulted in a profound inhibition of both the F1 fraction and LPS activities but 
      only in a partial inhibition of RU 41740 effects. These results strengthen the 
      hypothesis that different biochemical pathways are involved in the enhancement of 
      stimulated neutrophil functions by RU 41740.
FAU - el Abbouyi, A
AU  - el Abbouyi A
AD  - Laboratoire de Pharmacologie, Hopital Cochin, Paris, France.
FAU - Paul, J L
AU  - Paul JL
FAU - Roch-Arveiller, M
AU  - Roch-Arveiller M
FAU - Sarfati, G
AU  - Sarfati G
FAU - Giroud, J P
AU  - Giroud JP
FAU - Raichvarg, D
AU  - Raichvarg D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biol Response Mod
JT  - Journal of biological response modifiers
JID - 8219656
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Bacterial Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 68583-24-4 (glycoprotein, Klebsiella pneumoniae)
RN  - 9010-72-4 (Zymosan)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Bacterial Proteins/*pharmacology
MH  - Calcimycin/pharmacology
MH  - Chemical Fractionation
MH  - Glucuronidase/blood
MH  - Humans
MH  - Klebsiella pneumoniae/*analysis
MH  - Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects
MH  - Oxygen Consumption/drug effects
MH  - Superoxides/blood
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Zymosan/pharmacology
EDAT- 1989/12/01 00:00
MHDA- 1989/12/01 00:01
CRDT- 1989/12/01 00:00
PHST- 1989/12/01 00:00 [pubmed]
PHST- 1989/12/01 00:01 [medline]
PHST- 1989/12/01 00:00 [entrez]
PST - ppublish
SO  - J Biol Response Mod. 1989 Dec;8(6):656-64.