PMID- 25575056
OWN - NLM
STAT- MEDLINE
DCOM- 20150817
LR  - 20191210
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 31
IP  - 1
DP  - 2015 Jan
TI  - [Inhibition of S1PR2 activity down-regulates expressions of sphingosine kinase 1 
      and MCP-1 in rat glomerular mesangial cells under high glucose].
PG  - 36-9
AB  - OBJECTIVE: To investigate the effects of high glucose and the specific antagonist 
      JTE-013 of sphingosine-1-phosphate receptor 2 (S1PR2) on the expressions of 
      sphingosine kinase 1 (Sphk1), S1PR2 and monocyte chemoattractant protein-1 
      (MCP-1) in rat glomerular mesangial cells. METHODS: The cultured rat GMCs were 
      divided into four groups: normal glucose control group (NG, with 5.5 mmol/L 
      glucose), mannitol group (HM, with 5.5 mmol/L glucose and 24.5 mmol/L mannitol), 
      high glucose group (HG, with 30 mmol/L glucose), JTE-013 group (HJ, with 30 
      mmol/L glucose and 10 mumol/L JTE-013). The mRNA levels of SphK1, S1PR2 and MCP-1 
      were determined with real-time quantitative PCR in the cells at 0, 12, 24 and 48 
      hours, respectively, and the protein expression of MCP-1 in the supernatant was 
      determined with ELISA . RESULTS: Compared with those in normal glucose, the mRNAs 
      of SphK1 and S1PR2 in rat GMCs under high glucose were down-regulated at 12 hours 
      and were then up-regulated as time went on, and peaked at 48 hours. High glucose 
      significantly enhanced the mRNA expression of MCP-1 at 12 hours, and the 
      expression reached the highest levels at 24 hours, but decreased at 48 hours. The 
      protein expression of MCP-1 in rat GMCs time-dependently increased under high 
      glucose compared with that in NG. After GMCs were treated with 10 mumol/L JTE-013 
      before exposed to high glucose for 24 hours, the mRNA levels of SphK1, S1PR2 and 
      MCP-1 and the protein expression of MCP-1 significantly decreased compared with 
      those in HG. CONCLUSION: Inhibition of S1PR2 activity could down-regulate the 
      expressions of SphK1 and MCP-1 in rat GMCs under high glucose.
FAU - Lei, Sha
AU  - Lei S
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
FAU - Wang, Qiuyue
AU  - Wang Q
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
FAU - Lv, Chuan
AU  - Lv C
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
FAU - Wu, Can
AU  - Wu C
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
FAU - Yuan, Qin
AU  - Yuan Q
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
FAU - Han, Jinyu
AU  - Han J
AD  - Department of Endocrinology, First Affiliated Hospital, China Medical University, 
      Shenyang 110001, China.
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, Lysosphingolipid)
RN  - 0 (Sphingosine-1-Phosphate Receptors)
RN  - 0 (sphingosine-1-phosphate receptor-2, rat)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.- (sphingosine kinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Diabetic Nephropathies/enzymology/genetics/*metabolism
MH  - *Down-Regulation
MH  - Glucose/*metabolism
MH  - Humans
MH  - Mesangial Cells/enzymology/*metabolism
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics/*metabolism
MH  - Rats
MH  - Receptors, Lysosphingolipid/genetics/*metabolism
MH  - Sphingosine-1-Phosphate Receptors
EDAT- 2015/01/13 06:00
MHDA- 2015/08/19 06:00
CRDT- 2015/01/10 06:00
PHST- 2015/01/10 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Jan;31(1):36-9.