PMID- 25575683
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20190831
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
VI  - 53
IP  - 2
DP  - 2016 Mar
TI  - Migration and Phagocytic Ability of Activated Microglia During Post-natal 
      Development is Mediated by Calcium-Dependent Purinergic Signalling.
PG  - 944-954
LID - 10.1007/s12035-014-9064-3 [doi]
AB  - Microglia play an important role in synaptic pruning and controlled phagocytosis 
      of neuronal cells during developmental stages. However, the mechanisms that 
      regulate these functions are not completely understood. The present study was 
      designed to investigate the role of purinergic signalling in microglial migration 
      and phagocytic activity during post-natal brain development. One-day-old BALB/c 
      mice received lipopolysaccharide (LPS) and/or a purinergic analogue 
      (2-methylthioladenosine-5'-diphosphate; 2MeSADP), intracerebroventrically 
      (i.c.v.). Combined administration of LPS and 2MeSADP resulted in activation of 
      microglia as evident from increased expression of ionised calcium-binding adapter 
      molecule 1 (Iba1). Activated microglia showed increased expression of purinergic 
      receptors (P2Y2, P2Y6 and P2Y12). LPS either alone or in combination with 2MeSADP 
      induced the expression of Na(+)/Ca(2+) exchanger (NCX-1) and P/Q-type Ca(2+) 
      channels along with MARCKS-related protein (MRP), which is an integral component 
      of cell migration machinery. In addition, LPS and 2MeSADP administration induced 
      the expression of microglial CD11b and DAP12 (DNAX-activation protein 12), which 
      are known to be involved in phagocytosis of neurons during development. 
      Interestingly, administration of thapsigargin (TG), a specific Ca(2+)-ATPase 
      inhibitor of endoplasmic reticulum, prevented the LPS/2MeSADP-induced microglial 
      activation and migration by down-regulating the expression of Iba1 and MRP, 
      respectively. Moreover, TG also reduced the LPS/2MeSADP-induced expression of 
      CD11b/DAP12. Taken together, the findings reveal for the first time that 
      Ca(2+)-mediated purinergic receptors regulate the migration and phagocytic 
      ability of microglia during post-natal brain development.
FAU - Sunkaria, Aditya
AU  - Sunkaria A
AD  - Department of Biochemistry, Panjab University, Chandigarh, 160014, India.
FAU - Bhardwaj, Supriya
AU  - Bhardwaj S
AD  - Department of Dermatology, Postgraduate Institute of Medical Education & 
      Research, Chandigarh, India.
FAU - Halder, Avishek
AU  - Halder A
AD  - Department of Biochemistry, Panjab University, Chandigarh, 160014, India.
FAU - Yadav, Aarti
AU  - Yadav A
AD  - Department of Biochemistry, Panjab University, Chandigarh, 160014, India.
FAU - Sandhir, Rajat
AU  - Sandhir R
AD  - Department of Biochemistry, Panjab University, Chandigarh, 160014, India. 
      sandhir@pu.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150110
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CD11b Antigen)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Purinergic)
RN  - 0 (Tyrobp protein, mouse)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Adenosine Diphosphate/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/cytology
MH  - CD11b Antigen/metabolism
MH  - Calcium/*metabolism
MH  - *Cell Movement/drug effects
MH  - Cytoskeleton/drug effects/metabolism
MH  - Female
MH  - Intracellular Space/metabolism
MH  - Lipopolysaccharides
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Microglia/*cytology/drug effects/*metabolism
MH  - Models, Biological
MH  - *Phagocytosis/drug effects
MH  - Receptors, Purinergic/*metabolism
MH  - *Signal Transduction/drug effects
OTO - NOTNLM
OT  - Brain development
OT  - Calcium
OT  - DAP12
OT  - Microglia
OT  - Phagocytosis
OT  - Purinergic receptors
EDAT- 2015/01/13 06:00
MHDA- 2016/11/15 06:00
CRDT- 2015/01/11 06:00
PHST- 2014/10/01 00:00 [received]
PHST- 2014/12/09 00:00 [accepted]
PHST- 2015/01/11 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
AID - 10.1007/s12035-014-9064-3 [pii]
AID - 10.1007/s12035-014-9064-3 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2016 Mar;53(2):944-954. doi: 10.1007/s12035-014-9064-3. Epub 2015 
      Jan 10.