PMID- 25576290
OWN - NLM
STAT- MEDLINE
DCOM- 20150519
LR  - 20230202
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 46
IP  - 3
DP  - 2015 Mar
TI  - Primary mediastinal seminomas: a comprehensive immunohistochemical study with a 
      focus on novel markers.
PG  - 376-83
LID - S0046-8177(14)00478-X [pii]
LID - 10.1016/j.humpath.2014.11.009 [doi]
AB  - Primary mediastinal seminomas are unusual tumors that can present in a pure form 
      or as part of a mixed germ cell tumor. Contrary to testicular seminomas, little 
      is known about the expression of novel immunohistochemical markers in mediastinal 
      seminomas. This study investigates the immunohistochemical features of these 
      tumors with a focus on novel markers. Thirty-two cases of primary mediastinal 
      seminomas were reviewed; and representative whole-tissue sections were selected 
      for immunohistochemical studies using antibodies directed against high molecular 
      weight cytokeratin 5/6 (CK5/6), low molecular weight cytokeratin (CAM5.2), 
      octamer-binding transcription factor 3/4 (OCT3/4), spalt-like transcription 
      factor 4 (SALL4), GATA binding protein 3 (GATA-3), sry-related HMG box 2 (SOX2), 
      SOX17, human T cell leukemia/lymphoma 1 (TCL1), glypican 3, melanoma associated 
      antigen C2 (MAGEC2), and paired box gene 8 (Pax8). The percentage of positive 
      tumor cells as well as the intensity of staining was evaluated and scored. 
      Thirty-one cases (97%) expressed SOX17, whereas 29 cases (91%) were positive for 
      OCT3/4 and SALL4, respectively. Twenty-eight cases (88%) expressed MAGEC2 and 
      CAM5.2, respectively. Two cases (6%) were positive for Pax8, and a single case 
      (3%) was positive for TCL1. None of the cases stained with CK5/6, GATA-3, SOX2, 
      or glypican 3. Similar to testicular seminomas, mediastinal seminomas show 
      consistent expression of OCT3/4, SALL4, SOX17, and MAGEC2 and are negative for 
      SOX2, glypican 3, GATA-3, and CK5/6. Pax8 positivity is only inconsistently 
      identified in mediastinal seminomas. Contrary to their testicular counterparts, 
      mediastinal tumors show diffuse expression of low-molecular-weight cytokeratin in 
      up to 90% of cases and are commonly negative for TCL1. Although there is some 
      immunohistochemical overlap between testicular and mediastinal seminomas, 
      considerable differences also exist and should be acknowledged when dealing with 
      these tumors.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Weissferdt, Annikka
AU  - Weissferdt A
AD  - Department of Pathology, MD Anderson Cancer Center, Houston, TX 77030. Electronic 
      address: aweissferdt@mdanderson.org.
FAU - Rodriguez-Canales, Jaime
AU  - Rodriguez-Canales J
AD  - Department of Translational Molecular Pathology, MD Anderson Cancer Center, 
      Houston, TX 77030.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Translational Molecular Pathology, MD Anderson Cancer Center, 
      Houston, TX 77030.
FAU - Fujimoto, Junya
AU  - Fujimoto J
AD  - Department of Translational Molecular Pathology, MD Anderson Cancer Center, 
      Houston, TX 77030.
FAU - Wistuba, Ignacio I
AU  - Wistuba II
AD  - Department of Translational Molecular Pathology, MD Anderson Cancer Center, 
      Houston, TX 77030.
FAU - Moran, Cesar A
AU  - Moran CA
AD  - Department of Pathology, MD Anderson Cancer Center, Houston, TX 77030.
LA  - eng
GR  - P50 CA070907/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20141126
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (GPC3 protein, human)
RN  - 0 (Glypicans)
RN  - 0 (Keratin-5)
RN  - 0 (Keratin-6)
RN  - 0 (MAGEC2 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (PAX8 Transcription Factor)
RN  - 0 (PAX8 protein, human)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (SALL4 protein, human)
RN  - 0 (SOX17 protein, human)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
RN  - 0 (SOXF Transcription Factors)
RN  - 0 (TCL1A protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Antigens, Neoplasm/analysis
MH  - Biomarkers, Tumor/*analysis
MH  - GATA3 Transcription Factor/analysis
MH  - Glypicans/analysis
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratin-5/analysis
MH  - Keratin-6/analysis
MH  - Male
MH  - Mediastinal Neoplasms/*chemistry/pathology
MH  - Middle Aged
MH  - Neoplasm Proteins/analysis
MH  - Octamer Transcription Factor-3/analysis
MH  - PAX8 Transcription Factor
MH  - Paired Box Transcription Factors/analysis
MH  - Proto-Oncogene Proteins/analysis
MH  - SOXB1 Transcription Factors/analysis
MH  - SOXF Transcription Factors/analysis
MH  - Seminoma/*chemistry/pathology
MH  - Testicular Neoplasms/*chemistry/pathology
MH  - Transcription Factors/*analysis
MH  - Young Adult
OTO - NOTNLM
OT  - Germ cell tumor
OT  - Immunohistochemistry
OT  - Mediastinum
OT  - Seminoma
OT  - Thymus
EDAT- 2015/01/13 06:00
MHDA- 2015/05/20 06:00
CRDT- 2015/01/11 06:00
PHST- 2014/10/14 00:00 [received]
PHST- 2014/11/05 00:00 [revised]
PHST- 2014/11/12 00:00 [accepted]
PHST- 2015/01/11 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - S0046-8177(14)00478-X [pii]
AID - 10.1016/j.humpath.2014.11.009 [doi]
PST - ppublish
SO  - Hum Pathol. 2015 Mar;46(3):376-83. doi: 10.1016/j.humpath.2014.11.009. Epub 2014 
      Nov 26.