PMID- 25576296
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20221207
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 98
IP  - 1
DP  - 2015 Feb
TI  - Matrine protects neuro-axon from CNS inflammation-induced injury.
PG  - 124-30
LID - S0014-4800(15)00003-9 [pii]
LID - 10.1016/j.yexmp.2015.01.001 [doi]
AB  - Neuro-axonal injury in the central nervous system (CNS) is one of the major 
      pathological hallmarks of experimental autoimmune encephalomyelitis (EAE), an 
      experimental model of multiple sclerosis (MS). Matrine (MAT), a quinolizidine 
      alkaloid derived from the herb Radix Sophorae Flave, has recently been shown to 
      effectively suppress EAE through an anti-inflammatory mechanism. However, whether 
      MAT can also protect myelin/axons from damage is not known. In the present study 
      we show that, while untreated rats developed severe clinical disease, CNS 
      inflammatory demyelination, and axonal damage, these clinical and pathological 
      signs were significantly reduced by MAT treatment. Consistently, MAT treatment 
      reduced the concentration of myelin basic protein in serum and downregulated 
      expression of beta-amyloid (Abeta) and B-site APP cleaving enzyme 1 (BACE-1) in the 
      CNS. Further, the CNS of MAT-treated rats exhibited increased expression of 
      brain-derived neurotrophic factor (BDNF), an important factor for neuronal 
      survival and axonal growth. Together, these results demonstrate that MAT 
      effectively prevented neuro-axonal injury, which can likely be attributed to 
      inhibiting risk factors such as BACE-1 and upregulating neuroprotective factors 
      such as BDNF. We conclude that this novel natural reagent, MAT, which effectively 
      protects neuro-axons from CNS inflammation-induced damage, could be a potential 
      candidate for the treatment of neurodegenerative diseases such as MS.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Kan, Quan-Cheng
AU  - Kan QC
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Lv, Peng
AU  - Lv P
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Zhang, Xiao-Jian
AU  - Zhang XJ
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Xu, Yu-Ming
AU  - Xu YM
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Zhang, Guang-Xian
AU  - Zhang GX
AD  - Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, 
      USA. Electronic address: guang-xian.zhang@jefferson.edu.
FAU - Zhu, Lin
AU  - Zhu L
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China. Electronic address: zhulin66zhulin@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Alkaloids)
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Plant Extracts)
RN  - 0 (Quinolizines)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.46 (Bace1 protein, rat)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Amyloid Precursor Protein Secretases/genetics/metabolism
MH  - Animals
MH  - Aspartic Acid Endopeptidases/genetics/metabolism
MH  - Axons/*drug effects/metabolism/*pathology
MH  - Biomarkers/analysis
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cells, Cultured
MH  - Central Nervous System/*drug effects/metabolism/pathology
MH  - Encephalomyelitis, Autoimmune, Experimental/*drug therapy/immunology/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fabaceae/chemistry
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Inflammation/immunology/pathology/*prevention & control
MH  - Plant Extracts/*pharmacology
MH  - Quinolizines/*pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Matrines
OTO - NOTNLM
OT  - Axonal injury
OT  - EAE
OT  - MS
OT  - Matrine
EDAT- 2015/01/13 06:00
MHDA- 2015/05/23 06:00
CRDT- 2015/01/11 06:00
PHST- 2014/12/16 00:00 [received]
PHST- 2015/01/05 00:00 [accepted]
PHST- 2015/01/11 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - S0014-4800(15)00003-9 [pii]
AID - 10.1016/j.yexmp.2015.01.001 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2015 Feb;98(1):124-30. doi: 10.1016/j.yexmp.2015.01.001. Epub 
      2015 Jan 7.