PMID- 25576658
OWN - NLM
STAT- MEDLINE
DCOM- 20151123
LR  - 20181202
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 24
IP  - 2
DP  - 2015 Feb
TI  - IL-10 plays a pivotal role in anti-inflammatory effects of resveratrol in 
      activated microglia cells.
PG  - 369-376
LID - S1567-5769(14)00513-X [pii]
LID - 10.1016/j.intimp.2014.12.035 [doi]
AB  - The development of agents that can modulate microglial activation has been 
      suggested as one potential strategy for the treatment or prevention of 
      neurodegenerative diseases. Among these agents, resveratrol, with its 
      anti-inflammatory action, has been described to have neuroprotective effects. In 
      this paper we demonstrate that in LPS-stimulated microglia resveratrol 
      pretreatment reduced, in a dose-dependent manner, pro-inflammatory cytokines 
      IL-1beta, TNF-alpha and IL-6 mRNA expression and increased the release of 
      anti-inflammatory interleukin (IL)-10. Moreover, resveratrol pretreatment 
      up-regulated the phosphorylated forms of JAK1 and STAT3, as well as suppressor of 
      cytokine signaling (SOCS)3 protein expression in LPS activated cells, 
      demonstrating that the JAK-STAT signaling pathway is involved in the 
      anti-inflammatory effect exerted by resveratrol. By supplementing the cultures 
      with an IL-10 neutralizing antibody (IL-10NA) we obtained the opposite effect. 
      Taken together, these data allow us to conclude that the LPS-induced 
      pro-inflammatory response in microglial cells can be markedly reduced by 
      resveratrol, through IL-10 dependent up-regulation of SOCS3, requiring the 
      JAK-STAT signaling pathway.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Cianciulli, Antonia
AU  - Cianciulli A
AD  - Department of Biosciences, Biotechnologies and Biopharmaceutics, University of 
      Bari, Italy.
FAU - Dragone, Teresa
AU  - Dragone T
AD  - Department of Biosciences, Biotechnologies and Biopharmaceutics, University of 
      Bari, Italy.
FAU - Calvello, Rosa
AU  - Calvello R
AD  - Department of Biosciences, Biotechnologies and Biopharmaceutics, University of 
      Bari, Italy.
FAU - Porro, Chiara
AU  - Porro C
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
FAU - Trotta, Teresa
AU  - Trotta T
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
FAU - Lofrumento, Dario Domenico
AU  - Lofrumento DD
AD  - Department of Biological and Environmental Sciences and Technologies, Section of 
      Human Anatomy, University of Salento, Lecce, Italy.
FAU - Panaro, Maria Antonietta
AU  - Panaro MA
AD  - Department of Biosciences, Biotechnologies and Biopharmaceutics, University of 
      Bari, Italy. Electronic address: mariaantonietta.panaro@uniba.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Socs3 protein, mouse)
RN  - 0 (Stat3 protein, mouse)
RN  - 0 (Stilbenes)
RN  - 0 (Suppressor of Cytokine Signaling 3 Protein)
RN  - 0 (Suppressor of Cytokine Signaling Proteins)
RN  - EC 2.7.10.2 (Jak1 protein, mouse)
RN  - EC 2.7.10.2 (Janus Kinase 1)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cytokines/genetics/*metabolism
MH  - Janus Kinase 1/metabolism
MH  - Lipopolysaccharides
MH  - Mice
MH  - Microglia/*drug effects/metabolism
MH  - RNA, Messenger/metabolism
MH  - Resveratrol
MH  - STAT3 Transcription Factor/metabolism
MH  - Stilbenes/*pharmacology
MH  - Suppressor of Cytokine Signaling 3 Protein
MH  - Suppressor of Cytokine Signaling Proteins/metabolism
OTO - NOTNLM
OT  - IL-10
OT  - Inflammation
OT  - Microglia
OT  - Neurological diseases
OT  - Resveratrol
EDAT- 2015/01/13 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/01/11 06:00
PHST- 2014/10/06 00:00 [received]
PHST- 2014/12/19 00:00 [revised]
PHST- 2014/12/28 00:00 [accepted]
PHST- 2015/01/11 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S1567-5769(14)00513-X [pii]
AID - 10.1016/j.intimp.2014.12.035 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2015 Feb;24(2):369-376. doi: 10.1016/j.intimp.2014.12.035. 
      Epub 2015 Jan 7.