PMID- 25578421
OWN - NLM
STAT- MEDLINE
DCOM- 20151113
LR  - 20150220
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 126
DP  - 2015 Feb 1
TI  - Near infrared laser stimulation of human neural stem cells into neurons on 
      graphene nanomesh semiconductors.
PG  - 313-21
LID - S0927-7765(14)00713-9 [pii]
LID - 10.1016/j.colsurfb.2014.12.027 [doi]
AB  - Reduced graphene oxide nanomeshes (rGONMs), as p-type semiconductors with 
      band-gap energy of  approximately  1 eV, were developed and applied in near infrared (NIR) 
      laser stimulation of human neural stem cells (hNSCs) into neurons. The 
      biocompatibility of the rGONMs in growth of hNSCs was found similar to that of 
      the graphene oxide (GO) sheets. Proliferation of the hNSCs on the GONMs was 
      assigned to the excess oxygen functional groups formed on edge defects of the 
      GONMs, resulting in superhydrophilicity of the surface. Under NIR laser 
      stimulation, the graphene layers (especially the rGONMs) exhibited significant 
      cell differentiations, including more elongations of the cells and higher 
      differentiation of neurons than glia. The higher hNSC differentiation on the 
      rGONM than the reduced GO (rGO) was assigned to the stimulation effects of the 
      low-energy photoexcited electrons injected from the rGONM semiconductors into the 
      cells, while the high-energy photoelectrons of the rGO (as a zero band-gap 
      semiconductor) could suppress the cell proliferation and/or even cause cell 
      damages. Using conventional heating of the culture media up to  approximately  43  degrees C (the 
      temperature typically reached under the laser irradiation), no significant 
      differentiation was observed in dark. This further confirmed the role of 
      photoelectrons in the hNSC differentiation.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Akhavan, Omid
AU  - Akhavan O
AD  - Department of Physics, Sharif University of Technology, P.O. Box 11155-9161, 
      Tehran, Iran; Institute for Nanoscience and Nanotechnology, Sharif University of 
      Technology, P.O. Box 14588-89694, Tehran, Iran. Electronic address: 
      oakhavan@sharif.edu.
FAU - Ghaderi, Elham
AU  - Ghaderi E
AD  - Nanobiotechnology Research Lab., Division of Advanced Materials, Azadi Ave., 
      Tehran, Iran.
FAU - Shirazian, Soheil A
AU  - Shirazian SA
AD  - Department of Physics, Sharif University of Technology, P.O. Box 11155-9161, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141231
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Oxides)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Cells, Cultured
MH  - Graphite/*chemistry
MH  - Humans
MH  - *Lasers
MH  - Nanostructures/*chemistry
MH  - Neural Stem Cells/*cytology/*radiation effects
MH  - Neurons/*cytology/*radiation effects
MH  - Oxides/chemistry
MH  - Particle Size
MH  - *Semiconductors
MH  - Surface Properties
OTO - NOTNLM
OT  - Graphene nanomesh
OT  - NIR stimulation
OT  - Nanostructures
OT  - Neural differentiation
OT  - Stem cells
OT  - Tissue engineering
EDAT- 2015/01/13 06:00
MHDA- 2015/11/14 06:00
CRDT- 2015/01/13 06:00
PHST- 2014/09/29 00:00 [received]
PHST- 2014/12/10 00:00 [revised]
PHST- 2014/12/15 00:00 [accepted]
PHST- 2015/01/13 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/11/14 06:00 [medline]
AID - S0927-7765(14)00713-9 [pii]
AID - 10.1016/j.colsurfb.2014.12.027 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2015 Feb 1;126:313-21. doi: 
      10.1016/j.colsurfb.2014.12.027. Epub 2014 Dec 31.