PMID- 25579167
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR  - 20181113
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Linking)
VI  - 32
IP  - 2
DP  - 2015 Feb
TI  - Peperotetraphin inhibits the proliferation of human prostate cancer cells via 
      induction of cell cycle arrest and apoptosis.
PG  - 468
LID - 10.1007/s12032-014-0468-8 [doi]
AB  - Peperotetraphin (methyl rel-(1R,2S,3S)-2,3-bis(7-methoxy-1,3-benzodioxol-5-yl) 
      cyclobutanecarboxylate) was a novel cyclobutane-type norlignan, which was 
      isolated from the whole plant of Peperomia tetraphylla. In this study, we 
      explored its anti-tumor effect and the molecular mechanism in human prostate 
      cancer PC-3 cell lines. Firstly, cell viability was evaluated by Cell Counting 
      Kit (CCK-8) assay. The PC-3 cells were treated with increasing concentrations of 
      peperotetraphin for 24, 48 and 72 h, respectively. The results showed that 
      peperotetraphin inhibited the growth of PC-3 cell in a dose- and time-dependent 
      manner. Next, the cell cycle distributions were analyzed by flow cytometric 
      analysis (FCM), and the data suggested that peperotetraphin could significantly 
      induce cell cycle arrested at the G1-S phase transition. Then, the cell apoptosis 
      was detected by the terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) and annexin V-FITC/PI dual staining analysis, the data confirmed 
      apoptosis-inducing activity of peperotetraphin and the apoptosis rates increased 
      from 3.9 to 32.3 % when treated with increasing concentrations of peperotetraphin 
      from 0 to 50 microM. The expression levels of apoptosis-regulating protein caspase-3, 
      Bax and Bcl-2 were also analyzed by Western blot analysis. The results showed 
      that the expression levels of Bax and the activity of caspase-3 were upregulated, 
      whereas the expression levels of Bcl-2 were downregulated compared with those of 
      the control. These findings demonstrated that peperotetraphin exhibited effective 
      cell growth inhibition by inducing cancer to undergo G1 phase arrest and 
      apoptosis. The results suggested that peperotetraphin might have potential as 
      chemoprevention or anti-tumor agent to prostatic cancer.
FAU - Li, Yunzhi
AU  - Li Y
AD  - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230031, People's 
      Republic of China, yunzhili@live.com.
FAU - He, Ning
AU  - He N
FAU - Zhai, Chengwu
AU  - Zhai C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150113
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cyclobutanes)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Blotting, Western
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cyclobutanes/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Peperomia/*chemistry
MH  - Prostatic Neoplasms/*pathology
EDAT- 2015/01/13 06:00
MHDA- 2015/10/16 06:00
CRDT- 2015/01/13 06:00
PHST- 2014/12/13 00:00 [received]
PHST- 2014/12/16 00:00 [accepted]
PHST- 2015/01/13 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.1007/s12032-014-0468-8 [doi]
PST - ppublish
SO  - Med Oncol. 2015 Feb;32(2):468. doi: 10.1007/s12032-014-0468-8. Epub 2015 Jan 13.