PMID- 25580009
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20220408
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 457
IP  - 3
DP  - 2015 Feb 13
TI  - Modeled microgravity suppressed invasion and migration of human glioblastoma U87 
      cells through downregulating store-operated calcium entry.
PG  - 378-84
LID - S0006-291X(15)00005-4 [pii]
LID - 10.1016/j.bbrc.2014.12.120 [doi]
AB  - Glioblastoma is the most common brain tumor and is characterized with robust 
      invasion and migration potential resulting in poor prognosis. Previous 
      investigations have demonstrated that modeled microgravity (MMG) could decline 
      the cell proliferation and attenuate the metastasis potential in several cell 
      lines. In this study, we studied the effects of MMG on the invasion and migration 
      potentials of glioblastoma in human glioblastoma U87 cells. We found that MMG 
      stimulation significantly attenuated the invasion and migration potentials, 
      decreased thapsigargin (TG) induced store-operated calcium entry (SOCE) and 
      downregulated the expression of Orai1 in U87 cells. Inhibition of SOCE by 2-APB 
      or stromal interaction molecule 1 (STIM1) downregulation both mimicked the 
      effects of MMG on the invasion and migration potentials in U87 cells. 
      Furthermore, upregulation of Orai1 significantly weakened the effects of MMG on 
      the invasion and migration potentials in U87 cells. Therefore, these findings 
      indicated that MMG stimulation inhibited the invasion and migration potentials of 
      U87 cells by downregulating the expression of Orai1 and sequentially decreasing 
      the SOCE, suggesting that MMG might be a new potential therapeutic strategy in 
      glioblastoma treatment in the future.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Shi, Zi-xuan
AU  - Shi ZX
AD  - Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military 
      Medical University, Xi'an, 710032, PR China.
FAU - Rao, Wei
AU  - Rao W
AD  - Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 710032, PR China.
FAU - Wang, Huan
AU  - Wang H
AD  - Department of Dermatology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an, 710032, PR China.
FAU - Wang, Nan-ding
AU  - Wang ND
AD  - Department of Cardiology, Xi'an Traditional Chinese Medicine Hospital, Xi'an, 
      710032, PR China.
FAU - Si, Jing-wen
AU  - Si JW
AD  - Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military 
      Medical University, Xi'an, 710032, PR China.
FAU - Zhao, Jiao
AU  - Zhao J
AD  - Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military 
      Medical University, Xi'an, 710032, PR China.
FAU - Li, Jun-chang
AU  - Li JC
AD  - Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military 
      Medical University, Xi'an, 710032, PR China.
FAU - Wang, Zong-ren
AU  - Wang ZR
AD  - Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military 
      Medical University, Xi'an, 710032, PR China. Electronic address: 
      zongren@fmmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150109
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium Channels)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (ORAI1 Protein)
RN  - 0 (ORAI1 protein, human)
RN  - 0 (STIM1 protein, human)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Brain Neoplasms/*metabolism/pathology/*therapy
MH  - Calcium/*metabolism
MH  - Calcium Channels/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Down-Regulation
MH  - Glioblastoma/*metabolism/pathology/*therapy
MH  - Humans
MH  - Membrane Proteins/antagonists & inhibitors/genetics
MH  - Neoplasm Invasiveness
MH  - Neoplasm Proteins/antagonists & inhibitors/genetics
MH  - ORAI1 Protein
MH  - RNA Interference
MH  - Stromal Interaction Molecule 1
MH  - Up-Regulation
MH  - *Weightlessness Simulation
OTO - NOTNLM
OT  - Glioblastoma
OT  - Invasion
OT  - Microgravity
OT  - Migration
OT  - SOCE
EDAT- 2015/01/13 06:00
MHDA- 2015/07/15 06:00
CRDT- 2015/01/13 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2014/12/31 00:00 [accepted]
PHST- 2015/01/13 06:00 [entrez]
PHST- 2015/01/13 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S0006-291X(15)00005-4 [pii]
AID - 10.1016/j.bbrc.2014.12.120 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Feb 13;457(3):378-84. doi: 
      10.1016/j.bbrc.2014.12.120. Epub 2015 Jan 9.