PMID- 25582218
OWN - NLM
STAT- MEDLINE
DCOM- 20160226
LR  - 20191210
IS  - 1939-6376 (Electronic)
IS  - 1939-6368 (Linking)
VI  - 61
IP  - 3
DP  - 2015 Jun
TI  - A 24-chromosome FISH technique in preimplantation genetic diagnosis: validation 
      of the method.
PG  - 171-7
LID - 10.3109/19396368.2014.1002869 [doi]
AB  - Embryo screening for aneuploidy (AS) is part of preimplantation genetic 
      diagnostics (PGD) and is aimed at improving the efficiency of assisted 
      reproduction. Currently, several technologies, including the well-established 
      fluorescence in situ hybridization (FISH) technique, cover the screening of all 
      chromosomes in a single cell. This study evaluates a novel 24-chromosome FISH 
      technique protocol (FISH-24). A total of 337 embryos were analyzed using the 
      traditional 9-chromosome FISH technique (FISH-9) while 251 embryos were evaluated 
      using the new FISH-24 technique. Embryos deemed nontransferable on Day 3 were 
      cultured in vitro to Day 5 of development, then fixed and reanalyzed according to 
      the technique allocated to each treatment cycle (107 embryos analyzed by FISH-9 
      and 111 by FISH-24). The global error rate (discrepancy between Day 3 and Day 5 
      results for a single embryo) was 2.8% after FISH-9 and 3.6% after FISH-24, with a 
      p value of 0.95. Thus, we have established and validated a 24-chromosome 
      FISH-based single cell aneuploidy screening technique, showing that the error 
      rate obtained for FISH-24 is independent of the number of chromosomes analyzed 
      and equivalent to the error rate observed for FISH-9, as a useful tool for 
      chromosome segregation studies and clinical use.
FAU - Fernandez, Silvia F
AU  - Fernandez SF
AD  - Center for Embryo Medicine and.
FAU - Toro, Estefania
AU  - Toro E
FAU - Colomar, Ana
AU  - Colomar A
FAU - Lopez-Teijon, Marisa
AU  - Lopez-Teijon M
FAU - Velilla, Esther
AU  - Velilla E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20150113
PL  - England
TA  - Syst Biol Reprod Med
JT  - Systems biology in reproductive medicine
JID - 101464963
SB  - IM
MH  - *Chromosomes, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Preimplantation Diagnosis
OTO - NOTNLM
OT  - 24-chromosome analysis
OT  - FISH
OT  - aneuploidy screening (AS)
OT  - error rate
OT  - preimplantation genetic diagnosis (PGD)
EDAT- 2015/01/15 06:00
MHDA- 2016/02/27 06:00
CRDT- 2015/01/14 06:00
PHST- 2015/01/14 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2016/02/27 06:00 [medline]
AID - 10.3109/19396368.2014.1002869 [doi]
PST - ppublish
SO  - Syst Biol Reprod Med. 2015 Jun;61(3):171-7. doi: 10.3109/19396368.2014.1002869. 
      Epub 2015 Jan 13.