PMID- 25586037
OWN - NLM
STAT- MEDLINE
DCOM- 20161214
LR  - 20161230
IS  - 1549-7801 (Electronic)
IS  - 0738-8551 (Linking)
VI  - 36
IP  - 3
DP  - 2016
TI  - Prediction of melting temperatures in fluorescence in situ hybridization (FISH) 
      procedures using thermodynamic models.
PG  - 566-77
LID - 10.3109/07388551.2014.993589 [doi]
AB  - The thermodynamics and kinetics of DNA hybridization, i.e. the process of 
      self-assembly of one, two or more complementary nucleic acid strands, has been 
      studied for many years. The appearance of the nearest-neighbor model led to 
      several theoretical and experimental papers on DNA thermodynamics that provide 
      reasonably accurate thermodynamic information on nucleic acid duplexes and allow 
      estimation of the melting temperature. Because there are no thermodynamic models 
      specifically developed to predict the hybridization temperature of a probe used 
      in a fluorescence in situ hybridization (FISH) procedure, the melting temperature 
      is used as a reference, together with corrections for certain compounds that are 
      used during FISH. However, the quantitative relation between melting and 
      experimental FISH temperatures is poorly described. In this review, various 
      models used to predict the melting temperature for rRNA targets, for DNA 
      oligonucleotides and for nucleic acid mimics (chemically modified 
      oligonucleotides), will be addressed in detail, together with a critical 
      assessment of how this information should be used in FISH.
FAU - Fontenete, Silvia
AU  - Fontenete S
AD  - a Department of Chemical Engineering, Faculty of Engineering, LEPABE , University 
      of Porto , Porto , Portugal .
AD  - b Institute of Molecular Pathology and Immunology of the University of Porto , 
      Porto , Portugal .
AD  - c Department of Physics, Chemistry and Pharmacy , Nucleic Acid Center, University 
      of Southern Denmark , Odense M , Denmark , and.
AD  - d ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto , 
      Porto , Portugal.
FAU - Guimaraes, Nuno
AU  - Guimaraes N
AD  - a Department of Chemical Engineering, Faculty of Engineering, LEPABE , University 
      of Porto , Porto , Portugal .
AD  - b Institute of Molecular Pathology and Immunology of the University of Porto , 
      Porto , Portugal .
AD  - c Department of Physics, Chemistry and Pharmacy , Nucleic Acid Center, University 
      of Southern Denmark , Odense M , Denmark , and.
FAU - Wengel, Jesper
AU  - Wengel J
AD  - c Department of Physics, Chemistry and Pharmacy , Nucleic Acid Center, University 
      of Southern Denmark , Odense M , Denmark , and.
FAU - Azevedo, Nuno Filipe
AU  - Azevedo NF
AD  - a Department of Chemical Engineering, Faculty of Engineering, LEPABE , University 
      of Porto , Porto , Portugal .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20150114
PL  - England
TA  - Crit Rev Biotechnol
JT  - Critical reviews in biotechnology
JID - 8505177
RN  - 0 (Peptide Nucleic Acids)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *DNA/analysis/chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Models, Theoretical
MH  - Nucleic Acid Hybridization
MH  - Peptide Nucleic Acids/analysis/chemistry
MH  - Thermodynamics
MH  - *Transition Temperature
OTO - NOTNLM
OT  - DNA
OT  - locked nucleic acids
OT  - nucleic acid mimics
OT  - peptide nucleic acids
EDAT- 2015/01/15 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/01/15 06:00
PHST- 2015/01/15 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.3109/07388551.2014.993589 [doi]
PST - ppublish
SO  - Crit Rev Biotechnol. 2016;36(3):566-77. doi: 10.3109/07388551.2014.993589. Epub 
      2015 Jan 14.