PMID- 25586463
OWN - NLM
STAT- MEDLINE
DCOM- 20170711
LR  - 20210703
IS  - 1742-481X (Electronic)
IS  - 1742-4801 (Print)
IS  - 1742-4801 (Linking)
VI  - 13
IP  - 5
DP  - 2016 Oct
TI  - Increased expression of endosomal members of toll-like receptor family abrogates 
      wound healing in patients with type 2 diabetes mellitus.
PG  - 927-35
LID - 10.1111/iwj.12411 [doi]
AB  - The inflammatory phase of wound healing cascade is an important determinant of 
      the fate of the wound. Acute inflammation is necessary to initiate proper wound 
      healing, while chronic inflammation abrogates wound healing. Different endosomal 
      members of toll-like receptor (TLR) family initiate inflammatory signalling via a 
      range of different inflammatory mediators such as interferons, internal tissue 
      damaged-associated molecular patterns (DAMPs) and hyperactive effector T cells. 
      Sustained signalling of TLR9 and TLR7 contributes to chronic inflammation by 
      activating the plasmacytoid dendritic cells. Diabetic wounds are also 
      characterised by sustained inflammatory phase. The objective of this study was to 
      analyse the differential expression of endosomal TLRs in human diabetic wounds 
      compared with control wounds. We analysed the differential expression of TLR7 and 
      TLR9 both at transcriptional and translational levels in wounds of 84 patients 
      with type 2 diabetes mellitus (T2DM) and 6 control subjects without diabetes 
      using quantitative real-time polymerase chain reaction (RT-PCR), western blot and 
      immunohistochemistry. TLR7 and TLR9 were significantly up-regulated in wounds of 
      the patients with T2DM compared with the controls and were dependent on the 
      infection status of the diabetic wounds, and wounds with microbial infection 
      exhibited lower expression levels of endosomal TLRs. Altered endosomal TLR 
      expression in T2DM subjects might be associated with wound healing impairment.
CI  - (c) 2015 Medicalhelplines.com Inc and John Wiley & Sons Ltd.
FAU - Singh, Kanhaiya
AU  - Singh K
AD  - Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi, 
      221005, India.
FAU - Agrawal, Neeraj K
AU  - Agrawal NK
AD  - Department of Endocrinology and Metabolism, Institute of Medical Sciences, 
      Banaras Hindu University, Varanasi, 221005, India.
FAU - Gupta, Sanjeev K
AU  - Gupta SK
AD  - Department of General Surgery, Institute of Medical Sciences, Banaras Hindu 
      University, Varanasi, 221005, India.
FAU - Mohan, Gyanendra
AU  - Mohan G
AD  - Department of Pathology, Indian Railway Cancer Hospital and Research Center, 
      N.E.R., Varanasi, 221002, India.
FAU - Chaturvedi, Sunanda
AU  - Chaturvedi S
AD  - Department of Pathology, Indian Railway Cancer Hospital and Research Center, 
      N.E.R., Varanasi, 221002, India.
FAU - Singh, Kiran
AU  - Singh K
AD  - Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi, 
      221005, India. skiran@bhu.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20150114
PL  - England
TA  - Int Wound J
JT  - International wound journal
JID - 101230907
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR7 protein, human)
RN  - 0 (TLR9 protein, human)
RN  - 0 (Toll-Like Receptor 7)
RN  - 0 (Toll-Like Receptor 9)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*complications/*metabolism
MH  - Diabetic Foot/etiology/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/metabolism
MH  - Toll-Like Receptor 7/genetics/*metabolism
MH  - Toll-Like Receptor 9/genetics/*metabolism
MH  - Wound Healing/*physiology
PMC - PMC7949499
OTO - NOTNLM
OT  - Diabetic foot ulcer
OT  - T2DM
OT  - TLR7
OT  - TLR9
OT  - Toll-like receptors
OT  - Wound healing impairment
EDAT- 2015/01/15 06:00
MHDA- 2017/07/14 06:00
PMCR- 2015/01/14
CRDT- 2015/01/15 06:00
PHST- 2014/09/02 00:00 [received]
PHST- 2014/12/05 00:00 [revised]
PHST- 2014/12/09 00:00 [accepted]
PHST- 2015/01/15 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2015/01/14 00:00 [pmc-release]
AID - IWJ12411 [pii]
AID - 10.1111/iwj.12411 [doi]
PST - ppublish
SO  - Int Wound J. 2016 Oct;13(5):927-35. doi: 10.1111/iwj.12411. Epub 2015 Jan 14.