PMID- 25586548
OWN - NLM
STAT- MEDLINE
DCOM- 20151013
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Jan 14
TI  - Concomitant analysis of Helios and Neuropilin-1 as a marker to detect thymic 
      derived regulatory T cells in naive mice.
PG  - 7767
LID - 10.1038/srep07767 [doi]
LID - 7767
AB  - Regulatory T (Treg) cells are characterized by the expression of CD4, CD25 and 
      the intracellular Foxp3. However, these markers do not indicate whether Treg 
      cells are thymic derived Treg (tTreg) cells or peripherally induced Treg (pTreg) 
      cells. Recently, Helios and Neuropilin-1 (Nrp1) has been reported as potential 
      markers for tTreg cells. Herein, we used flow cytometry to examine the proportion 
      of CD4(+)CD8(-)CD25(+) Treg cells expressing Helios, Nrp1 and Foxp3 in thymus, 
      pancreatic draining lymph nodes (PDLNs) and spleen of CD-1 mice, and thymus of 
      NOD and C57BL/6 mice. The frequency of Helios(+) cells was higher than that of 
      Nrp1(+) cells in CD4(+)CD8(-)CD25(+) and CD4(+)CD8(-)CD25(+)Foxp3(+) Treg cells 
      in thymus. Interestingly, the proportion of IL-10(+), Ebi3(+)and CTLA-4(+) cells 
      was higher in Helios(+) than Nrp1(+) tTreg cells. The anti-apoptotic activity of 
      Helios(+) tTreg cells was higher in thymus compared to Nrp1(+) tTreg cells. Nrp1 
      seems to be expressed at a later developmental stage compared to Helios and 
      Foxp3. Furthermore, the expression of Nrp1 in CD4(+)CD25(+) T cells of younger 
      mice did not increase after stimulating them in vitro with anti-CD3 and -CD28. 
      Thus, under these conditions, Helios could be considered a more reliable marker 
      for distinguishing tTreg cells from pTreg cells than Nrp1.
FAU - Singh, Kailash
AU  - Singh K
AD  - Department of Medical Cell Biology, BMC, Uppsala University, Uppsala, Sweden.
FAU - Hjort, Marcus
AU  - Hjort M
AD  - Department of Medical Cell Biology, BMC, Uppsala University, Uppsala, Sweden.
FAU - Thorvaldson, Lina
AU  - Thorvaldson L
AD  - Department of Medical Cell Biology, BMC, Uppsala University, Uppsala, Sweden.
FAU - Sandler, Stellan
AU  - Sandler S
AD  - Department of Medical Cell Biology, BMC, Uppsala University, Uppsala, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150114
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ebi3 protein, mouse)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Transcription Factors)
RN  - 0 (Zfpn1a2 protein, mouse)
RN  - 130068-27-8 (Interleukin-10)
RN  - 144713-63-3 (Neuropilin-1)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - CTLA-4 Antigen/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Interleukin-10/metabolism
MH  - Lymph Nodes/cytology
MH  - Lymphocyte Count
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NOD
MH  - Minor Histocompatibility Antigens
MH  - Neuropilin-1/*metabolism
MH  - Receptors, Cytokine/metabolism
MH  - Spleen/cytology
MH  - T-Lymphocytes, Regulatory/*metabolism
MH  - Thymus Gland/*cytology
MH  - Transcription Factors/*metabolism
PMC - PMC4293597
EDAT- 2015/01/15 06:00
MHDA- 2015/10/16 06:00
PMCR- 2015/01/14
CRDT- 2015/01/15 06:00
PHST- 2014/08/28 00:00 [received]
PHST- 2014/12/16 00:00 [accepted]
PHST- 2015/01/15 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
PHST- 2015/01/14 00:00 [pmc-release]
AID - srep07767 [pii]
AID - 10.1038/srep07767 [doi]
PST - epublish
SO  - Sci Rep. 2015 Jan 14;5:7767. doi: 10.1038/srep07767.