PMID- 25587068
OWN - NLM
STAT- MEDLINE
DCOM- 20150911
LR  - 20220321
IS  - 1533-3450 (Electronic)
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 26
IP  - 7
DP  - 2015 Jul
TI  - MicroRNA-687 Induced by Hypoxia-Inducible Factor-1 Targets Phosphatase and Tensin 
      Homolog in Renal Ischemia-Reperfusion Injury.
PG  - 1588-96
LID - 10.1681/ASN.2014050463 [doi]
AB  - Ischemia-reperfusion injury contributes to tissue damage and organ failure in 
      clinical settings, but the underlying mechanism remains elusive and effective 
      therapies are still lacking. Here, we identified microRNA 687 (miR-687) as a key 
      regulator and therapeutic target in renal ischemia-reperfusion injury. We show 
      that miR-687 is markedly upregulated in the kidney during renal 
      ischemia-reperfusion in mice and in cultured kidney cells during hypoxia. MiR-687 
      induction under these conditions was mediated by hypoxia-inducible factor-1 
      (HIF-1). Upon induction in vitro, miR-687 repressed the expression of phosphatase 
      and tensin homolog (PTEN) and facilitated cell cycle progression and apoptosis. 
      Blockade of miR-687 preserved PTEN expression and attenuated cell cycle 
      activation and renal apoptosis, resulting in protection against kidney injury in 
      mice. Collectively, these results unveil a novel HIF-1/miR-687/PTEN signaling 
      pathway in ischemia-reperfusion injury that may be targeted for therapy.
CI  - Copyright (c) 2015 by the American Society of Nephrology.
FAU - Bhatt, Kirti
AU  - Bhatt K
AD  - Department of Cellular Biology and Anatomy, Georgia Regents University and 
      Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia;
FAU - Wei, Qingqing
AU  - Wei Q
AD  - Department of Cellular Biology and Anatomy, Georgia Regents University and 
      Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia;
FAU - Pabla, Navjotsingh
AU  - Pabla N
AD  - Department of Cellular Biology and Anatomy, Georgia Regents University and 
      Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia;
FAU - Dong, Guie
AU  - Dong G
AD  - Department of Cellular Biology and Anatomy, Georgia Regents University and 
      Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia;
FAU - Mi, Qing-Sheng
AU  - Mi QS
AD  - Departments of Dermatology and Internal Medicine, Henry Ford Health System, 
      Detroit, Michigan;
FAU - Liang, Mingyu
AU  - Liang M
AD  - Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin;
FAU - Mei, Changlin
AU  - Mei C
AD  - Department of Nephrology, Changzheng Hospital, Second Military Medical 
      University, Shanghai, China; and.
FAU - Dong, Zheng
AU  - Dong Z
AD  - Department of Cellular Biology and Anatomy, Georgia Regents University and 
      Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia; Department of 
      Nephrology, The Second Xiangya Hospital, Central South University, Changsha, 
      Hunan, China zdong@gru.edu.
LA  - eng
GR  - I01 BX000319/BX/BLRD VA/United States
GR  - R01 DK058831/DK/NIDDK NIH HHS/United States
GR  - R01 DK087843/DK/NIDDK NIH HHS/United States
GR  - R01 HL121233/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150113
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (MicroRNAs)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Tensins)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (Pten protein, mouse)
SB  - IM
CIN - J Am Soc Nephrol. 2015 Jul;26(7):1478-80. PMID: 25587069
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Northern
MH  - Disease Models, Animal
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Kidney Diseases/*physiopathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/metabolism
MH  - Microfilament Proteins/*genetics/metabolism
MH  - PTEN Phosphohydrolase/*genetics
MH  - Random Allocation
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Reperfusion Injury/*physiopathology
MH  - Signal Transduction
MH  - Tensins
MH  - Up-Regulation
PMC - PMC4483585
OTO - NOTNLM
OT  - acute renal failure
OT  - cell death
OT  - hypoxia
OT  - ischemia-reperfusion
OT  - renal injury
EDAT- 2015/01/15 06:00
MHDA- 2015/09/12 06:00
PMCR- 2016/07/01
CRDT- 2015/01/15 06:00
PHST- 2014/05/12 00:00 [received]
PHST- 2014/09/12 00:00 [accepted]
PHST- 2015/01/15 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - ASN.2014050463 [pii]
AID - 2014050463 [pii]
AID - 10.1681/ASN.2014050463 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2015 Jul;26(7):1588-96. doi: 10.1681/ASN.2014050463. Epub 2015 
      Jan 13.