PMID- 25587335
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150114
LR  - 20201001
IS  - 1735-0328 (Print)
IS  - 1726-6890 (Electronic)
IS  - 1726-6882 (Linking)
VI  - 13
IP  - 4
DP  - 2014 Fall
TI  - Determination of some B Vitamins in Sour Cherry Juice Using Dispersive 
      Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography.
PG  - 1437-45
AB  - Dispersive liquid-liquid microextraction method (DLLME) combined with 
      high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used 
      to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry 
      juice. This method was rapid, simple and sensitive. Separation was accomplished 
      using a C18 column. The optimum chromatographic conditions were found to be: 
      mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid 
      water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. 
      The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced 
      by factors such as: additional salt effect, the kind and volume of disperser and 
      extraction solvents. In this research, the limit of detection (LOD) and 
      quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for 
      nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations 
      (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the 
      observed concentration range was obtained with the value of R(2)>0.996 for the 
      target vitamins. This method was successfully applied to the sour cherry juice 
      samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this 
      research, was a local variety of the sour cherry with large stone, double 
      flowers, double fruits, dark red skin and dark red juice. This variety was 
      identified in high altitude areas of Isfahan province after five years of study, 
      since 2005, by Agricultural and Natural Resources Research Center of Isfahan.
FAU - Parsaei, Parvin
AU  - Parsaei P
AD  - Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, 
      Iran.
FAU - Bahmaei, Manouchehr
AU  - Bahmaei M
AD  - Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, 
      Iran.
FAU - Ghannadi, AliReza
AU  - Ghannadi A
AD  - School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical 
      Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Iran J Pharm Res
JT  - Iranian journal of pharmaceutical research : IJPR
JID - 101208407
PMC - PMC4232812
OTO - NOTNLM
OT  - Dispersive liquid-liquid microextraction (DLLME)
OT  - High performance liquid chromatography-ultraviolet detector (HPLC-UV)
OT  - Nicotinamide
OT  - Pyridoxine
OT  - Thiamine
EDAT- 2015/01/15 06:00
MHDA- 2015/01/15 06:01
PMCR- 2014/09/01
CRDT- 2015/01/15 06:00
PHST- 2015/01/15 06:00 [entrez]
PHST- 2015/01/15 06:00 [pubmed]
PHST- 2015/01/15 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - ijpr-13-1437 [pii]
PST - ppublish
SO  - Iran J Pharm Res. 2014 Fall;13(4):1437-45.