PMID- 25588515
OWN - NLM
STAT- MEDLINE
DCOM- 20160129
LR  - 20190108
IS  - 1471-2091 (Electronic)
IS  - 1471-2091 (Linking)
VI  - 16
DP  - 2015 Jan 15
TI  - Mechano-growth factor accelerates the proliferation and osteogenic 
      differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway.
PG  - 1
LID - 10.1186/s12858-015-0031-z [doi]
LID - 1
AB  - BACKGROUND: Mesenchymal stem cells (MSCs) can differentiate into chondroblasts, 
      adipocytes, or osteoblasts under appropriate stimulation. Mechano-growth factor 
      (MGF) reportedly displays a neuroprotective effect in cerebral regions that were 
      exposed to ischemia and is expressed in stromal cells of the eutopic endometrium 
      and in glandular cells of the ectopic endometrium. RESULTS: This study sought to 
      understand the potential involvement of phosphatidylinositol-3-kinase 
      (PI3K)/protein kinase B (AKT) in MGF-induced growth of rabbit MSCs (rMSCs). We 
      applied various concentrations of MGF to cultured rMSCs and observed the growth 
      rate of the cells, the changes in the phosphorylation state of AKT and mammalian 
      target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and 
      osteocalcin. We found that the growth and osteogenic differentiation of 
      MGF-induced rMSCs were promoted primarily by phosphorylated AKT, and that this 
      phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor. 
      CONCLUSION: Our study suggests that MGF promotes the growth and osteogenic 
      differentiation of rMSCs primarily through the PI3K/AKT pathway.
FAU - Tong, Yanxiang
AU  - Tong Y
AD  - Department of Orthopedic, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China. 1059151081@qq.com.
FAU - Feng, Wei
AU  - Feng W
AD  - The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 
      China. weifang@hotmail.com.
FAU - Wu, Yimin
AU  - Wu Y
AD  - The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 
      China. yimingwu@126.com.
FAU - Lv, Huicheng
AU  - Lv H
AD  - The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 
      China. huicheng122@126.com.
FAU - Jia, Yanfei
AU  - Jia Y
AD  - The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 
      China. yufei3@yahoo.com.
FAU - Jiang, Dianming
AU  - Jiang D
AD  - Department of Orthopedic, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China. jiangdianmingtyx@163.com.
LA  - eng
PT  - Journal Article
DEP - 20150115
PL  - England
TA  - BMC Biochem
JT  - BMC biochemistry
JID - 101084098
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/drug effects
MH  - Insulin-Like Growth Factor I/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Osteoblasts/cytology/drug effects
MH  - Osteogenesis/*drug effects
MH  - Phosphatidylinositol 3-Kinase/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Rabbits
MH  - Signal Transduction/*drug effects
MH  - Time Factors
PMC - PMC4299473
EDAT- 2015/01/16 06:00
MHDA- 2016/01/30 06:00
PMCR- 2015/01/15
CRDT- 2015/01/16 06:00
PHST- 2014/12/10 00:00 [received]
PHST- 2015/01/07 00:00 [accepted]
PHST- 2015/01/16 06:00 [entrez]
PHST- 2015/01/16 06:00 [pubmed]
PHST- 2016/01/30 06:00 [medline]
PHST- 2015/01/15 00:00 [pmc-release]
AID - s12858-015-0031-z [pii]
AID - 31 [pii]
AID - 10.1186/s12858-015-0031-z [doi]
PST - epublish
SO  - BMC Biochem. 2015 Jan 15;16:1. doi: 10.1186/s12858-015-0031-z.