PMID- 25590304
OWN - NLM
STAT- MEDLINE
DCOM- 20151014
LR  - 20181202
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 29
IP  - 1
DP  - 2015 Jan-Feb
TI  - Serum level of interleukin-21 is elevated in chronic rhinosinusitis.
PG  - e1-6
LID - 10.2500/ajra.2015.29.4117 [doi]
AB  - BACKGROUND: Chronic rhinosinusitis (CRS) is an inflammatory disease of the 
      sinuses and mucosa with unclear pathogenesis. Interleukin (IL)-21 is mainly 
      expressed in activated cluster of differentiation (CD)4(+) T cells and has potent 
      regulatory effects on the immune system. OBJECTIVE: This study is to determine 
      whether IL-21 in the blood is correlated with CRS. METHODS: The blood samples 
      from CRS patients and normal controls were analyzed in correlation with clinical 
      features. The eosinophil percentage was counted, and serum levels of total 
      immunoglobulin E (IgE) and IL-21 were analyzed by enzyme-linked immunosorbent 
      assay (ELISA). In addition, IL-21 and interferon (IFN)-gamma secreted from stimulated 
      peripheral blood mononuclear cells (PBMCs) were measured by ELISA, and their mRNA 
      expression levels were analyzed by real-time quantitative polymerase chain 
      reaction (RT-qPCR). Disease severity was scored based on computed tomography (CT) 
      scan, nasal endoscopy, and global osteitis scoring scale (GOSS). RESULTS: A total 
      of 55 CRS and 37 healthy subjects were recruited. The average levels of serum 
      total IgE were 20 kU/L in normal group, 290 kU/L in CRS with nasal polys 
      (CRSwNP), and 187 kU/L in CRS without nasal polys (CRSsNP). IL-21 levels were 28 
      pg/mL in normal group, 54 pg/mL in CRSwNP, and 71 pg/mL in CRSsNP. Both IgE and 
      IL-21 were significantly elevated in both CRS patient subgroups. However, no 
      significant difference was found between these two patient subgroups. The serum 
      IL-21 levels correlated well with the disease severity in the patients. In 
      addition, the secreted IL-21 was enhanced significantly in the patient's PBMCs 
      stimulated by phytohemagglutin (PHA). CONCLUSION: IL-21 could be a target for 
      diagnosis and treatment of CRS.
FAU - Chao, Pin-Zhir
AU  - Chao PZ
AD  - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan.
FAU - Hsieh, Ming-Shium
AU  - Hsieh MS
FAU - Lee, Fei-Peng
AU  - Lee FP
FAU - Chen, Shao-Yi
AU  - Chen SY
FAU - Cheng, Chao-Wen
AU  - Cheng CW
FAU - Chang, Hui-Wen
AU  - Chang HW
FAU - Lin, Yun-Tien
AU  - Lin YT
FAU - Ting, Lai-Lei
AU  - Ting LL
FAU - Lin, Yung-Feng
AU  - Lin YF
FAU - Chen, Chien-Ho
AU  - Chen CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Interleukins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - MKM3CA6LT1 (interleukin-21)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Interferon-gamma/biosynthesis
MH  - Interleukins/*blood
MH  - Male
MH  - Middle Aged
MH  - Rhinitis/*immunology
MH  - Sinusitis/*immunology
EDAT- 2015/01/16 06:00
MHDA- 2015/10/16 06:00
CRDT- 2015/01/16 06:00
PHST- 2015/01/16 06:00 [entrez]
PHST- 2015/01/16 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.2500/ajra.2015.29.4117 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2015 Jan-Feb;29(1):e1-6. doi: 10.2500/ajra.2015.29.4117.