PMID- 2559068
OWN - NLM
STAT- MEDLINE
DCOM- 19900314
LR  - 20190828
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 93
IP  - 2
DP  - 1989
TI  - cGMP immunocytochemistry in aorta, kidney, retina and brain tissues of the rat 
      after perfusion with nitroprusside.
PG  - 143-8
AB  - The distribution of cyclic guanosine 3',5' monophosphate (cGMP) producing cells 
      in various organs of the rat were studied immunocytochemically using antibodies 
      raised against formaldehyde-fixed cGMP. Sodium nitroprusside (SNP), a direct 
      activator of guanylate cyclase and vasodilator, was used to enhance cGMP levels. 
      In order to reach all organs optimally, whole body perfusion was performed using 
      a modified Krebs-Ringer buffer at 37 degrees C, aerated with 5% CO2/95% O2, also 
      containing isobutyl methyl xanthine (IBMX); a phosphodiesterase inhibitor. After 
      15-min pre-perfusion, SNP was added to the perfusate, followed by fast fixation 
      with ice-cold 4% paraformaldehyde-phosphate buffer. After vehicle perfusion, only 
      the retina showed cGMP immunoreactivity in the photoreceptor and ganglion layer, 
      while other organs lacked cGMP immunoreactivity. After 15-min perfusion with SNP 
      (10 microM), enhanced cGMP immunostaining was seen in smooth muscles of the 
      aorta, amacrine-like cells in the retina, glomeruli of the kidney cortex, blood 
      vessels in the dura mater, as well as cells in the pineal and in the median 
      eminence. The results indicate that the distribution and the reactivity of cGMP 
      producing cells, situated outside the blood brain barrier, can be studied by 
      immunocytochemistry after pharmacological manipulations of the intact tissue with 
      a nitrovasodilator using whole body perfusion.
FAU - Berkelmans, H S
AU  - Berkelmans HS
AD  - Department of Pharmacology, Duphar B.V., Weesp, The Netherlands.
FAU - Schipper, J
AU  - Schipper J
FAU - Hudson, L
AU  - Hudson L
FAU - Steinbusch, H W
AU  - Steinbusch HW
FAU - de Vente, J
AU  - de Vente J
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 169D1260KM (Nitroprusside)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Aorta/*analysis/drug effects
MH  - *Brain Chemistry/drug effects
MH  - Cyclic GMP/*analysis
MH  - Kidney/*analysis/drug effects/ultrastructure
MH  - Male
MH  - Nitroprusside/pharmacology
MH  - Perfusion/methods
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Retina/*analysis/drug effects/ultrastructure
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1007/BF00315967 [doi]
PST - ppublish
SO  - Histochemistry. 1989;93(2):143-8. doi: 10.1007/BF00315967.