PMID- 25590820
OWN - NLM
STAT- MEDLINE
DCOM- 20160113
LR  - 20150320
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 286
DP  - 2015 Apr 9
TI  - Enhanced tolerance and remediation to mixed contaminates of PCBs and 2,4-DCP by 
      transgenic alfalfa plants expressing the 2,3-dihydroxybiphenyl-1,2-dioxygenase.
PG  - 269-75
LID - S0304-3894(14)01033-4 [pii]
LID - 10.1016/j.jhazmat.2014.12.049 [doi]
AB  - Polychlorinated biphenyls (PCBs) and 2,4-dichlorophenol (2,4-DCP) generally led 
      to mixed contamination of soils as a result of commercial and agricultural 
      activities. Their accumulation in the environment poses great risks to human and 
      animal health. Therefore, the effective strategies for disposal of these 
      pollutants are urgently needed. In this study, genetic engineering to enhance 
      PCBs/2,4-DCP phytoremediation is a focus. We cloned the 
      2,3-dihydroxybiphenyl-1,2-dioxygenase (BphC.B) from a soil metagenomic library, 
      which is the key enzyme of aerobic catabolism of a variety of aromatic compounds, 
      and then it was expressed in alfalfa driven by CaMV 35S promoter using 
      Agrobacterium-mediated transformation. Transgenic line BB11 was selected out 
      through PCR, Western blot analysis and enzyme activity assays. Its disposal and 
      tolerance to both PCBs and 2,4-DCP were examined. The tolerance capability of 
      transgenic line BB11 towards complex contaminants of PCBs/2,4-DCP significantly 
      increased compared with non-transgenic plants. Strong dissipation of PCBs and 
      high removal efficiency of 2,4-DCP were exhibited in a short time. It was 
      confirmed expressing BphC.B would be a feasible strategy to help achieving 
      phytoremediation in mixed contaminated soils with PCBs and 2,4-DCP.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wang, Yan
AU  - Wang Y
AD  - Key Laboratory of Groud Water Resources and Environment of the Ministry of 
      Education, College of Environment and Resources, Jilin University, 2519 Jiefang 
      Road, Changchun, Jilin 130021, People's Republic of China; College of Plant 
      Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, People's 
      Republic of China.
FAU - Ren, Hejun
AU  - Ren H
AD  - Key Laboratory of Groud Water Resources and Environment of the Ministry of 
      Education, College of Environment and Resources, Jilin University, 2519 Jiefang 
      Road, Changchun, Jilin 130021, People's Republic of China. Electronic address: 
      renhejun@126.com.
FAU - Pan, Hongyu
AU  - Pan H
AD  - College of Plant Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 
      130062, People's Republic of China.
FAU - Liu, Jinliang
AU  - Liu J
AD  - College of Plant Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 
      130062, People's Republic of China.
FAU - Zhang, Lanying
AU  - Zhang L
AD  - Key Laboratory of Groud Water Resources and Environment of the Ministry of 
      Education, College of Environment and Resources, Jilin University, 2519 Jiefang 
      Road, Changchun, Jilin 130021, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141227
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Chlorophenols)
RN  - 0 (Soil Pollutants)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.13.11.- (Dioxygenases)
RN  - EC 1.13.11.39 (2,3-dihydroxybiphenyl oxygenase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Chlorophenols/*analysis/metabolism
MH  - Cloning, Molecular
MH  - Dioxygenases/genetics/*metabolism
MH  - Medicago sativa/enzymology/genetics/growth & development/*metabolism
MH  - Plants, Genetically Modified/enzymology/growth & development/*metabolism
MH  - Polychlorinated Biphenyls/*analysis/metabolism
MH  - Seeds/enzymology/growth & development/metabolism
MH  - Soil Microbiology
MH  - Soil Pollutants/*analysis/metabolism
OTO - NOTNLM
OT  - 2,3-Dihydroxybiphenyl-1,2-dioxygenase
OT  - 2,4-Dichlorophenol
OT  - Phytoremediation
OT  - Polychlorinated biphenyls
OT  - Transgenic alfalfas
EDAT- 2015/01/16 06:00
MHDA- 2016/01/14 06:00
CRDT- 2015/01/16 06:00
PHST- 2014/07/23 00:00 [received]
PHST- 2014/11/14 00:00 [revised]
PHST- 2014/12/24 00:00 [accepted]
PHST- 2015/01/16 06:00 [entrez]
PHST- 2015/01/16 06:00 [pubmed]
PHST- 2016/01/14 06:00 [medline]
AID - S0304-3894(14)01033-4 [pii]
AID - 10.1016/j.jhazmat.2014.12.049 [doi]
PST - ppublish
SO  - J Hazard Mater. 2015 Apr 9;286:269-75. doi: 10.1016/j.jhazmat.2014.12.049. Epub 
      2014 Dec 27.