PMID- 25595300
OWN - NLM
STAT- MEDLINE
DCOM- 20151125
LR  - 20240322
IS  - 1095-9130 (Electronic)
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 75
DP  - 2015 Mar
TI  - Methods to study chaperone-mediated autophagy.
PG  - 133-40
LID - S1046-2023(15)00007-9 [pii]
LID - 10.1016/j.ymeth.2015.01.003 [doi]
AB  - Chaperone-mediated autophagy (CMA) is a multistep process that involves selective 
      degradation and digestion of a pool of soluble cytosolic proteins in lysosomes. 
      Cytosolic substrates are selectively identified and targeted by chaperones to 
      lysosomes where they are subsequently translocated into the organelle lumen 
      through a dedicated CMA-associated lysosomal membrane receptor/translocation 
      complex. CMA contributes to maintaining a functional proteome, through 
      elimination of altered proteins, and participates in the cellular energetic 
      balance through amino acid recycling. Defective or dysfunctional CMA has been 
      associated with human pathologies such as neurodegeneration, cancer, 
      immunodeficiency or diabetes, increasing the overall interest in methods to 
      monitor this selective autophagic pathway. Here, we describe approaches used to 
      study CMA in different experimental models.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Patel, Bindi
AU  - Patel B
AD  - Department of Developmental and Molecular Biology, Albert Einstein College of 
      Medicine, Bronx, NY 10461, USA; Institute for Aging Studies, Albert Einstein 
      College of Medicine, Bronx, NY 10461, USA.
FAU - Cuervo, Ana Maria
AU  - Cuervo AM
AD  - Department of Developmental and Molecular Biology, Albert Einstein College of 
      Medicine, Bronx, NY 10461, USA; Institute for Aging Studies, Albert Einstein 
      College of Medicine, Bronx, NY 10461, USA. Electronic address: 
      ana-maria.cuervo@einstein.yu.edu.
LA  - eng
GR  - P01 AG031782/AG/NIA NIH HHS/United States
GR  - P30 DK041296/DK/NIDDK NIH HHS/United States
GR  - R01 DK098408/DK/NIDDK NIH HHS/United States
GR  - R37 AG021904/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150114
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Autophagy/*genetics
MH  - Humans
MH  - Lysosomes/genetics/metabolism
MH  - Molecular Biology/*methods
MH  - Molecular Chaperones/*genetics/metabolism
MH  - Proteolysis
PMC - PMC4355229
MID - NIHMS655752
OTO - NOTNLM
OT  - Autophagy
OT  - Fluorescent reporters
OT  - Lysosomes
OT  - Organelle isolation
OT  - Proteolysis
EDAT- 2015/01/18 06:00
MHDA- 2015/12/15 06:00
PMCR- 2016/03/01
CRDT- 2015/01/18 06:00
PHST- 2014/11/12 00:00 [received]
PHST- 2014/12/30 00:00 [revised]
PHST- 2015/01/06 00:00 [accepted]
PHST- 2015/01/18 06:00 [entrez]
PHST- 2015/01/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - S1046-2023(15)00007-9 [pii]
AID - 10.1016/j.ymeth.2015.01.003 [doi]
PST - ppublish
SO  - Methods. 2015 Mar;75:133-40. doi: 10.1016/j.ymeth.2015.01.003. Epub 2015 Jan 14.