PMID- 25595362
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20181202
IS  - 1532-6500 (Electronic)
IS  - 1058-2746 (Print)
IS  - 1058-2746 (Linking)
VI  - 24
IP  - 3
DP  - 2015 Mar
TI  - Identification of shoulder osteoarthritis biomarkers: comparison between 
      shoulders with and without osteoarthritis.
PG  - 382-90
LID - S1058-2746(14)00661-2 [pii]
LID - 10.1016/j.jse.2014.11.039 [doi]
AB  - BACKGROUND: The biologic factors associated with shoulder osteoarthritis (OA) 
      have not been elucidated. The purpose of this study was to investigate 
      osteoarthritic biomarkers of the shoulder. To our knowledge, this is the first 
      study to analyze shoulder cartilage for OA-associated genes and to examine human 
      shoulder cartilage for a possible biomarker, connexin 43 (Cx43). MATERIALS AND 
      METHODS: Cartilage from 16 osteoarthritic and 10 nonosteoarthritic humeral heads 
      was assessed for expression of the following genes by real-time polymerase chain 
      reaction: types I, II, and X collagen; matrix metalloproteinases (MMPs); tissue 
      inhibitors of MMP (TIMPs); interleukins; versican; cyclooxygenase 2 (Cox-2); 
      inducible nitric oxide synthase (iNOS); tumor necrosis factor alpha (TNF-alpha); 
      aggrecanase 2 (ADAMTS5); and Cx43. RESULTS: In osteoarthritic shoulders, Cx43, 
      Cox-2, versican, collagen type I, ADAMTS5, MMP-3, and TNF-alpha expressions were 
      significantly increased compared with controls. TIMP-3 and iNOS trended toward 
      significance, with robust expression in osteoarthritic shoulders and low 
      expression in nonosteoarthritic shoulders. In osteoarthritic shoulders, gene 
      expression of Cx43, ADAMTS5, collagen type I, Cox-2, versican, and TIMP-3 showed 
      predominance (85-, 33-, 13-, 12-, 11.5-, and 3-fold increases, respectively) 
      relative to nonosteoarthritic controls. Spearman correlation analysis showed 
      significant correlations between Cx43 and collagen (types I, II, and X), MMP-9, 
      TIMP-2 and TIMP-3, versican, Cox-2, iNOS, and ADAMTS5. CONCLUSIONS: Certain genes 
      are markedly upregulated in osteoarthritic shoulders compared with 
      nonosteoarthritic shoulders, with Cx43, Cox-2, versican, collagen type I, 
      ADAMTS5, MMP-3, and TNF-alpha expression being significantly increased. These genes 
      might be useful biomarkers for examining shoulder OA. CLINICAL RELEVANCE: 
      Identification of osteoarthritic biomarkers can help us better understand 
      shoulder OA and build the foundation for future research on disease progression 
      and treatments.
CI  - Copyright (c) 2015 Journal of Shoulder and Elbow Surgery Board of Trustees. 
      Published by Elsevier Inc. All rights reserved.
FAU - Casagrande, Danielle
AU  - Casagrande D
AD  - Department of Orthopaedics, University of Maryland, Baltimore, MD, USA.
FAU - Stains, Joseph P
AU  - Stains JP
AD  - Department of Orthopaedics, University of Maryland, Baltimore, MD, USA. 
      Electronic address: jstains@umoa.umm.edu.
FAU - Murthi, Anand M
AU  - Murthi AM
AD  - Department of Orthopaedics, University of Maryland, Baltimore, MD, USA.
LA  - eng
GR  - R01 AR052719/AR/NIAMS NIH HHS/United States
GR  - R01 AR063631/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20150113
PL  - United States
TA  - J Shoulder Elbow Surg
JT  - Journal of shoulder and elbow surgery
JID - 9206499
RN  - 0 (Biomarkers)
RN  - 0 (Connexin 43)
RN  - 0 (GJA1 protein, human)
RN  - 0 (Interleukins)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (VCAN protein, human)
RN  - 126968-45-4 (Versicans)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (ADAMTS5 Protein)
RN  - EC 3.4.24.- (ADAMTS5 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - ADAM Proteins/biosynthesis
MH  - ADAMTS5 Protein
MH  - Adult
MH  - Aged
MH  - Biomarkers/*metabolism
MH  - Cartilage, Articular/*chemistry
MH  - Collagen/biosynthesis
MH  - Connexin 43/*analysis
MH  - Cyclooxygenase 2/biosynthesis
MH  - Female
MH  - Humans
MH  - Humeral Head/*chemistry
MH  - Interleukins/biosynthesis
MH  - Male
MH  - Matrix Metalloproteinases/biosynthesis
MH  - Middle Aged
MH  - Nitric Oxide Synthase Type II/biosynthesis
MH  - Osteoarthritis/genetics/*metabolism
MH  - Shoulder Joint/*chemistry
MH  - Tissue Inhibitor of Metalloproteinases/biosynthesis
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Up-Regulation
MH  - Versicans/biosynthesis
PMC - PMC4331258
MID - NIHMS655899
OTO - NOTNLM
OT  - Shoulder
OT  - biomarkers
OT  - connexin 43
OT  - osteoarthritis
EDAT- 2015/01/18 06:00
MHDA- 2015/07/07 06:00
PMCR- 2016/03/01
CRDT- 2015/01/18 06:00
PHST- 2013/12/30 00:00 [received]
PHST- 2014/11/12 00:00 [revised]
PHST- 2014/11/15 00:00 [accepted]
PHST- 2015/01/18 06:00 [entrez]
PHST- 2015/01/18 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - S1058-2746(14)00661-2 [pii]
AID - 10.1016/j.jse.2014.11.039 [doi]
PST - ppublish
SO  - J Shoulder Elbow Surg. 2015 Mar;24(3):382-90. doi: 10.1016/j.jse.2014.11.039. 
      Epub 2015 Jan 13.