PMID- 25595765
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 81
IP  - 6
DP  - 2015 Mar
TI  - Profiling microbial communities in manganese remediation systems treating coal 
      mine drainage.
PG  - 2189-98
LID - 10.1128/AEM.03643-14 [doi]
AB  - Water discharging from abandoned coal mines can contain extremely high manganese 
      levels. Removing this metal is an ongoing challenge. Passive Mn(II) removal beds 
      (MRBs) contain microorganisms that oxidize soluble Mn(II) to insoluble Mn(III/IV) 
      minerals, but system performance is unpredictable. Using amplicon pyrosequencing, 
      we profiled the bacterial, fungal, algal, and archaeal communities in four MRBs, 
      performing at different levels, in Pennsylvania to determine whether they 
      differed among MRBs and from surrounding soil and to establish the relative 
      abundance of known Mn(II) oxidizers. Archaea were not detected; PCRs with 
      archaeal primers returned only nontarget bacterial sequences. Fungal taxonomic 
      profiles differed starkly between sites that remove the majority of influent Mn 
      and those that do not, with the former being dominated by Ascomycota (mostly 
      Dothideomycetes) and the latter by Basidiomycota (almost entirely 
      Agaricomycetes). Taxonomic profiles for the other groups did not differ 
      significantly between MRBs, but operational taxonomic unit-based analyses showed 
      significant clustering by MRB with all three groups (P < 0.05). Soil samples 
      clustered separately from MRBs in all groups except fungi, whose soil samples 
      clustered loosely with their respective MRB. Known Mn(II) oxidizers accounted for 
      a minor proportion of bacterial sequences (up to 0.20%) but a greater proportion 
      of fungal sequences (up to 14.78%). MRB communities are more diverse than 
      previously thought, and more organisms may be capable of Mn(II) oxidation than 
      are currently known.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Chaput, Dominique L
AU  - Chaput DL
AUID- ORCID: 0000-0002-9736-2619
AD  - Department of Mineral Sciences, National Museum of Natural History, Smithsonian 
      Institution, Washington, DC, USA.
FAU - Hansel, Colleen M
AU  - Hansel CM
AD  - Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic 
      Institution, Woods Hole, Massachusetts, USA.
FAU - Burgos, William D
AU  - Burgos WD
AD  - Department of Civil and Environmental Engineering, The Pennsylvania State 
      University, University Park, Pennsylvania, USA.
FAU - Santelli, Cara M
AU  - Santelli CM
AD  - Department of Mineral Sciences, National Museum of Natural History, Smithsonian 
      Institution, Washington, DC, USA santellic@si.edu.
LA  - eng
SI  - BioProject/PRJNA229802
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150116
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Industrial Waste)
RN  - 42Z2K6ZL8P (Manganese)
SB  - IM
MH  - Archaea
MH  - Bacteria/classification/*isolation & purification/metabolism
MH  - *Biota
MH  - Coal Mining
MH  - *Environmental Microbiology
MH  - Fungi
MH  - *Industrial Waste
MH  - Manganese/*metabolism
MH  - Microalgae/classification/*isolation & purification/metabolism
MH  - Molecular Sequence Data
MH  - Pennsylvania
MH  - Sequence Analysis, DNA
PMC - PMC4345392
EDAT- 2015/01/18 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/09/01
CRDT- 2015/01/18 06:00
PHST- 2015/01/18 06:00 [entrez]
PHST- 2015/01/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - AEM.03643-14 [pii]
AID - 03643-14 [pii]
AID - 10.1128/AEM.03643-14 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2015 Mar;81(6):2189-98. doi: 10.1128/AEM.03643-14. Epub 
      2015 Jan 16.