PMID- 25595974
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20161125
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 289
DP  - 2015 Mar 19
TI  - Activation of NF-kappaB signaling pathway in HSV-1-induced mouse facial palsy: 
      Possible relation to therapeutic effect of glucocorticoids.
PG  - 251-61
LID - S0306-4522(15)00036-6 [pii]
LID - 10.1016/j.neuroscience.2014.12.062 [doi]
AB  - It has been documented that infection of herpes simplex virus type 1 (HSV-1) 
      contributes to the initiation of Bell's palsy. However, the exact mechanisms 
      responsible for this disorder have not been fully elucidated to date. A mouse 
      model of facial palsy induced by HSV-1 provides an opportunity to investigate the 
      alteration in activities of nuclear factor-kappa B (NF-kappaB) and its consequent 
      effect on two key inflammatory factors, i.e., tumor necrosis factor (TNF)-alpha and 
      cyclooxygenase-2 (COX-2), as well as the effect of glucocorticoids (GCs) in this 
      work. I-kappa B (IkappaB)-alpha phosphorylation and NF-kappaB nuclear translocation were 
      measured by western blotting, and NF-kappaB/DNA binding activity was assessed by 
      electrophoretic mobility shift assay (EMSA). Results showed the IkappaB-alpha 
      phosphorylation and degradation as well as NF-kappaB activation in a time-dependent 
      manner. The expression of TNF-alpha and COX-2 were determined by real-time polymerase 
      chain reaction (PCR), western blotting and/or enzyme-linked immunosorbent assay 
      (ELISA) respectively. Concomitant with the activation, the expression and 
      secretion of TNF-alpha and COX-2 were rapidly induced in HSV-1-infected paralyzed 
      mice. Conversely, the activation of NF-kappaB and up-regulation of TNF-alpha and COX-2 
      were blocked by pretreatment with NF-kappaB inhibitor pyrrolidine dithiocarbamate 
      (PDTC) before being inoculated with HSV-1 to mice. In addition, GCs inhibited the 
      nuclear translocation and DNA binding activity of NF-kappaB via inhibiting IkappaB-alpha 
      degradation. Meanwhile, TNF-alpha production and COX-2 expression were significantly 
      reduced by GCs. In conclusion, HSV-1 inoculation induced the activation of NF-kappaB, 
      expression and secretion of TNF-alpha and COX-2 in the facial paralyzed mice, while, 
      glucocorticoid effectively down-regulated TNF-alpha and COX-2 expression in 
      HSV-1-induced paralyzed mice.
CI  - Copyright (c) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Liu, W
AU  - Liu W
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China; Institute of Eye and ENT, Provincial Hospital Affiliated to 
      Shandong University, Jinan, PR China.
FAU - Fan, Z
AU  - Fan Z
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China.
FAU - Han, Y
AU  - Han Y
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China.
FAU - Xu, L
AU  - Xu L
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China.
FAU - Wang, M
AU  - Wang M
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China.
FAU - Zhang, D
AU  - Zhang D
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China.
FAU - Mao, Y
AU  - Mao Y
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China; Institute of Eye and ENT, Provincial Hospital Affiliated to 
      Shandong University, Jinan, PR China.
FAU - Li, J
AU  - Li J
AD  - Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye 
      and ENT, Provincial Hospital Affiliated to Shandong University, Jinan, PR China. 
      Electronic address: lijianfeng@hotmail.com.
FAU - Wang, H
AU  - Wang H
AD  - Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong 
      University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, 
      Jinan, PR China; Institute of Eye and ENT, Provincial Hospital Affiliated to 
      Shandong University, Jinan, PR China. Electronic address: 
      wang.hb7585@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150113
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Glucocorticoids)
RN  - 0 (Hormone Antagonists)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrrolidines)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Thiocarbamates)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - 320T6RNW1F (Mifepristone)
RN  - 5GMR90S4KN (Methylprednisolone Hemisuccinate)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.10 (Chuk protein, mouse)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage
MH  - Brain Stem/drug effects/*physiopathology
MH  - Cyclooxygenase 2/metabolism
MH  - Disease Models, Animal
MH  - Facial Paralysis/*drug therapy/*physiopathology
MH  - Glucocorticoids/*administration & dosage
MH  - Herpesvirus 1, Human
MH  - Hormone Antagonists/pharmacology
MH  - I-kappa B Kinase/metabolism
MH  - Male
MH  - Methylprednisolone Hemisuccinate/administration & dosage
MH  - Mice, Inbred BALB C
MH  - Mifepristone/pharmacology
MH  - NF-kappa B/*metabolism
MH  - Pyrrolidines/pharmacology
MH  - Receptors, Glucocorticoid/antagonists & inhibitors/metabolism
MH  - Signal Transduction/drug effects
MH  - Thiocarbamates/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - cyclooxygenase-2
OT  - facial palsy
OT  - glucocorticoid
OT  - herpes simplex virus type 1
OT  - nuclear factor-kappa B
OT  - tumor necrosis factor-alpha
EDAT- 2015/01/18 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/01/18 06:00
PHST- 2014/09/11 00:00 [received]
PHST- 2014/12/23 00:00 [revised]
PHST- 2014/12/24 00:00 [accepted]
PHST- 2015/01/18 06:00 [entrez]
PHST- 2015/01/18 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0306-4522(15)00036-6 [pii]
AID - 10.1016/j.neuroscience.2014.12.062 [doi]
PST - ppublish
SO  - Neuroscience. 2015 Mar 19;289:251-61. doi: 10.1016/j.neuroscience.2014.12.062. 
      Epub 2015 Jan 13.