PMID- 25598707
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150119
LR  - 20200930
IS  - 1476-9255 (Print)
IS  - 1476-9255 (Electronic)
IS  - 1476-9255 (Linking)
VI  - 11
IP  - 1
DP  - 2014
TI  - miRNA-146 negatively regulates the production of pro-inflammatory cytokines via 
      NF-kappaB signalling in human gingival fibroblasts.
PG  - 38
LID - 10.1186/s12950-014-0038-z [doi]
LID - 38
AB  - OBJECTIVE: In human gingival fibroblasts (HGFs), TLR4 recognises 
      Pathogen-associated molecular patterns (PAMPs), such as LPS, and subsequently 
      activates downstream signals that lead to the production of pro-inflammatory 
      cytokines. The aim of this study was to explore the mechanisms of LPS-induced 
      miRNA-146 regulation of TLR4 signals in HGFs. MATERIALS AND METHODS: HGFs were 
      treated with Porphyromonas gingivalis (P.g) LPS, the cells were harvested, and 
      kinase phosphorylation levels were detected by western blot. Selective 
      pharmacological inhibitors and agonists were used to block or activate the 
      relevant kinases, miRNA-146a/b expression levels were detected by real-time PCR, 
      and IL-1, IL-6, and TNF-alpha production were measured by enzyme-linked immunosorbent 
      assays (ELISA). A luciferase reporter plasmid containing miRNA-146a/b promoter 
      was tested in terms of p50/p65 regulation. RESULTS: After P.g LPS treatment, 
      NF-kappaB and Erk1/2 were strongly activated in HGFs. miRNA-146a/b, IL-1, IL-6 and 
      TNF-alpha levels were down-regulated when NF-kappaB inhibitor was used. p50/p65 strongly 
      activated miRNA-146a/b promoter as measured with the luciferase assay. 
      CONCLUSION: In TLR4 signalling in HGFs, both miRNA-146a and miRNA-146b are 
      downstream targets of NF-kappaB, but not of AP-1 signalling. miRNA-146a/b expression 
      was specifically dependent on NF-kappaB but not Erk1/2 or JNK signalling.
FAU - Xie, Yu-Feng
AU  - Xie YF
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Shu, Rong
AU  - Shu R
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Jiang, Shao-Yun
AU  - Jiang SY
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Song, Zhong-Chen
AU  - Song ZC
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Guo, Qiu-Man
AU  - Guo QM
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Dong, Jia-Chen
AU  - Dong JC
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
FAU - Lin, Zhi-Kai
AU  - Lin ZK
AD  - Department of Periodontology, College of Stomatology, Ninth People's Hospital, 
      School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of 
      Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011 China ; Shanghai Key 
      Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20141206
PL  - England
TA  - J Inflamm (Lond)
JT  - Journal of inflammation (London, England)
JID - 101232234
PMC - PMC4297434
OTO - NOTNLM
OT  - Human gingival fibroblasts
OT  - NF-kappaB
OT  - Pro-inflammatory cytokines
OT  - miRNA-146
EDAT- 2015/01/20 06:00
MHDA- 2015/01/20 06:01
PMCR- 2014/12/06
CRDT- 2015/01/20 06:00
PHST- 2014/01/23 00:00 [received]
PHST- 2014/11/15 00:00 [accepted]
PHST- 2015/01/20 06:00 [entrez]
PHST- 2015/01/20 06:00 [pubmed]
PHST- 2015/01/20 06:01 [medline]
PHST- 2014/12/06 00:00 [pmc-release]
AID - 38 [pii]
AID - 10.1186/s12950-014-0038-z [doi]
PST - epublish
SO  - J Inflamm (Lond). 2014 Dec 6;11(1):38. doi: 10.1186/s12950-014-0038-z. 
      eCollection 2014.