PMID- 25599671
OWN - NLM
STAT- MEDLINE
DCOM- 20160909
LR  - 20200108
IS  - 1934-8258 (Electronic)
IS  - 1934-8258 (Linking)
VI  - 84
DP  - 2015 Jan 20
TI  - Preparation of Cells from Formalin-Fixed, Paraffin-Embedded Tissue for Use in 
      Fluorescence In Situ Hybridization (FISH) Experiments.
PG  - 8.8.1-8.8.10
LID - 10.1002/0471142905.hg0808s84 [doi]
AB  - Numerical and structural chromosome abnormalities can be accurately detected in 
      cells from archived tissues using fluorescence in situ hybridization (FISH). This 
      unit describes two common approaches to performing FISH in formalin-fixed, 
      paraffin-embedded tissue. The first approach utilizes 4 to 6 mum tissue sections 
      in cases for which preserving tissue morphology is necessary, and the second 
      involves extraction of intact nuclei from 50-mum tissue sections. To interpret 
      FISH results using 4 to 6 mum sections, an adequate number of nuclei must be 
      evaluated to perform statistical analysis. Evaluation of 30 to 50 nuclei from the 
      single-cell suspension generally gives an interpretable result.
CI  - Copyright (c) 2015 John Wiley & Sons, Inc.
FAU - Weremowicz, Stanislawa
AU  - Weremowicz S
AD  - CAMD-Cytogenetics, Department of Pathology, Brigham and Women's Hospital, Boston, 
      Massachusetts.
LA  - eng
PT  - Journal Article
DEP - 20150120
PL  - United States
TA  - Curr Protoc Hum Genet
JT  - Current protocols in human genetics
JID - 101287858
RN  - 0 (DNA Probes)
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Cell Fractionation/instrumentation/methods
MH  - Cell Nucleus/chemistry/ultrastructure
MH  - Cell Separation/instrumentation/*methods
MH  - *Chromosome Aberrations
MH  - DNA Probes/chemical synthesis
MH  - Fixatives
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Microtomy/instrumentation/methods
MH  - Paraffin Embedding/instrumentation/*methods
MH  - Single-Cell Analysis/instrumentation/methods
MH  - Tissue Fixation/*methods
OTO - NOTNLM
OT  - FISH
OT  - archived tissue
OT  - interphase cytogenetics
EDAT- 2015/01/21 06:00
MHDA- 2016/09/10 06:00
CRDT- 2015/01/21 06:00
PHST- 2015/01/21 06:00 [entrez]
PHST- 2015/01/21 06:00 [pubmed]
PHST- 2016/09/10 06:00 [medline]
AID - 10.1002/0471142905.hg0808s84 [doi]
PST - epublish
SO  - Curr Protoc Hum Genet. 2015 Jan 20;84:8.8.1-8.8.10. doi: 
      10.1002/0471142905.hg0808s84.