PMID- 25603813
OWN - NLM
STAT- MEDLINE
DCOM- 20151006
LR  - 20221207
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 79
IP  - 1
DP  - 2015
TI  - Tomato extract suppresses the production of proinflammatory mediators induced by 
      interaction between adipocytes and macrophages.
PG  - 82-7
LID - 10.1080/09168451.2014.962472 [doi]
AB  - Obese adipose tissue is characterized by enhanced macrophage infiltration. A loop 
      involving monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha 
      (TNFalpha) between adipocytes and macrophages establishes a vicious cycle that 
      augments inflammatory changes and insulin resistance in obese adipose tissue. 
      Tomatoes, one of the most popular crops worldwide, contain many beneficial 
      phytochemicals that improve obesity-related diseases such as diabetes. Some of 
      them have also been reported to have anti-inflammatory properties. In this study, 
      we focused on the potential protective effects of phytochemicals in tomatoes on 
      inflammation. We screened fractions of tomato extract using nitric oxide (NO) 
      assay in lipopolysaccharide (LPS)-stimulated RAW264 macrophages. One fraction, 
      RF52, significantly inhibited NO production in LPS-stimulated RAW264 macrophages. 
      Furthermore, RF52 significantly decreased MCP-1 and TNFalpha productions. The 
      coculture of 3T3-L1 adipocytes and RAW264 macrophages markedly enhanced MCP-1, 
      TNFalpha, and NO productions compared with the control cultures; however, the 
      treatment with RF52 inhibited the production of these proinflammatory mediators. 
      These results suggest that RF52 from tomatoes may have the potential to suppress 
      inflammation by inhibiting the production of NO or proinflammatory cytokines 
      during the interaction between adipocytes and macrophages.
FAU - Kim, Young-il
AU  - Kim YI
AD  - a Laboratory of Molecular Function of Food, Division of Food Science and 
      Biotechnology, Graduate School of Agriculture , Kyoto University , Uji, Kyoto , 
      Japan.
FAU - Mohri, Shinsuke
AU  - Mohri S
FAU - Hirai, Shizuka
AU  - Hirai S
FAU - Lin, Shan
AU  - Lin S
FAU - Goto, Tsuyoshi
AU  - Goto T
FAU - Ohyane, Chie
AU  - Ohyane C
FAU - Sakamoto, Tomoya
AU  - Sakamoto T
FAU - Takahashi, Haruya
AU  - Takahashi H
FAU - Shibata, Daisuke
AU  - Shibata D
FAU - Takahashi, Nobuyuki
AU  - Takahashi N
FAU - Kawada, Teruo
AU  - Kawada T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*drug effects/metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Cell Line
MH  - Chemokine CCL2/antagonists & inhibitors/biosynthesis
MH  - Coculture Techniques
MH  - Lipopolysaccharides/antagonists & inhibitors/pharmacology
MH  - Solanum lycopersicum/*chemistry
MH  - Macrophage Activation
MH  - Macrophages/cytology/*drug effects/metabolism
MH  - Mice
MH  - Nitric Oxide/antagonists & inhibitors/biosynthesis
MH  - Phytochemicals/*pharmacology
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/biosynthesis
OTO - NOTNLM
OT  - adipocyte
OT  - macrophage
OT  - monocyte chemoattractant protein-1(MCP-1)
OT  - tomato
OT  - tumor necrosis factor-alpha (TNFalpha)
EDAT- 2015/01/22 06:00
MHDA- 2015/10/07 06:00
CRDT- 2015/01/22 06:00
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/10/07 06:00 [medline]
AID - 10.1080/09168451.2014.962472 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2015;79(1):82-7. doi: 10.1080/09168451.2014.962472.