PMID- 25604821
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20150121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 857
DP  - 2015 Feb 1
TI  - Tween 20-stabilized gold nanoparticles combined with adenosine 
      triphosphate-BODIPY conjugates for the fluorescence detection of adenosine with 
      more than 1000-fold selectivity.
PG  - 64-70
LID - S0003-2670(14)01400-7 [pii]
LID - 10.1016/j.aca.2014.11.038 [doi]
AB  - This study describes the development of a simple, enzyme-free, label-free, 
      sensitive, and selective system for detecting adenosine based on the use of Tween 
      20-stabilized gold nanoparticles (Tween 20-AuNPs) as an efficient fluorescence 
      quencher for boron dipyrromethene-conjugated adenosine 5'-triphosphate 
      (BODIPY-ATP) and as a recognition element for adenosine. BODIPY-ATP can interact 
      with Tween 20-AuNPs through the coordination between the adenine group of 
      BODIPY-ATP and Au atoms on the NP surface, thereby causing the fluorescence 
      quenching of BODIPY-ATP through the nanometal surface energy transfer (NSET) 
      effect. When adenosine attaches to the NP surface, the attached adenosine 
      exhibits additional electrostatic attraction to BODIPY-ATP. As a result, the 
      presence of adenosine enhances the efficiency of AuNPs in fluorescence quenching 
      of BODIPY-ATP. The AuNP-induced fluorescence quenching of BODIPY-ATP 
      progressively increased with an increase in the concentration of adenosine; the 
      detection limit at a signal-to-noise ratio of 3 for adenosine was determined to 
      be 60nM. The selectivity of the proposed system was more than 1000-fold for 
      adenosine over any adenosine analogs and other nucleotides. The proposed system 
      combined with a phenylboronic acid-containing column was successfully applied to 
      the determination of adenosine in urine.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Hung, Szu-Ying
AU  - Hung SY
AD  - Department of Chemistry, National Sun Yat-sen University, Taiwan.
FAU - Shih, Ya-Chen
AU  - Shih YC
AD  - Department of Chemistry, National Sun Yat-sen University, Taiwan.
FAU - Tseng, Wei-Lung
AU  - Tseng WL
AD  - Department of Chemistry, National Sun Yat-sen University, Taiwan; School of 
      Pharmacy, College of Pharmacy, Kaohsiung Medical University, Taiwan; Center for 
      Nanoscience and Nanotechnology, National Sun Yat-sen University, Taiwan; Center 
      for Stem Cell Research, Kaohsiung Medical University, Taiwan. Electronic address: 
      tsengwl@mail.nsysu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141203
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
RN  - 0 (Boron Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polysorbates)
RN  - 7440-57-5 (Gold)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*analysis/urine
MH  - Adenosine Triphosphate/*chemistry
MH  - Adult
MH  - Boron Compounds/*chemistry
MH  - Female
MH  - Fluorescent Dyes
MH  - Gold
MH  - Humans
MH  - Limit of Detection
MH  - Nanoparticles/*chemistry
MH  - Polysorbates/*chemistry
MH  - Sensitivity and Specificity
MH  - Signal-To-Noise Ratio
MH  - Spectrometry, Fluorescence/instrumentation/methods
OTO - NOTNLM
OT  - Adenosine
OT  - Fluorescence
OT  - Nanoparticles
OT  - Sensor
EDAT- 2015/01/22 06:00
MHDA- 2015/09/17 06:00
CRDT- 2015/01/22 06:00
PHST- 2014/08/14 00:00 [received]
PHST- 2014/11/26 00:00 [revised]
PHST- 2014/11/30 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - S0003-2670(14)01400-7 [pii]
AID - 10.1016/j.aca.2014.11.038 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2015 Feb 1;857:64-70. doi: 10.1016/j.aca.2014.11.038. Epub 2014 
      Dec 3.