PMID- 25605482
OWN - NLM
STAT- MEDLINE
DCOM- 20151208
LR  - 20181202
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 15
DP  - 2015 Jan 21
TI  - Human leukocyte antigen class I and class II alleles are associated with 
      susceptibility and resistance in borderline leprosy patients from Southeast 
      Brazil.
PG  - 22
LID - 10.1186/s12879-015-0751-0 [doi]
LID - 22
AB  - BACKGROUND: Evidence suggests that human leukocyte antigen (HLA) alleles 
      influence the host immune response against Mycobacterium leprae. However, the 
      association between HLA alleles and borderline (B) leprosy has not been studied. 
      The aim of this study was to determine whether HLA class I and II molecules are 
      associated with susceptibility or resistance to B leprosy including 
      borderline-tuberculoid (BT), borderline-borderline (BB), and 
      borderline-lepromatous (BL). METHODS: DNA was obtained by the salting-out 
      technique from the blood samples of 202 patients with B leprosy and 478 control 
      subjects. HLA class I (A*, B*, and C* loci) and class II (DRB1* and DQB1* loci) 
      genotypes were determined by polymerase chain reaction amplification and reverse 
      hybridization with sequence-specific oligonucleotide probes and sequence-specific 
      primers. RESULTS: The case-controlled analysis results showed a significant 
      association between B leprosy and HLA-C*05 (5.94% vs. 14.02%; p = 0.002, OR = 
      0.38, 95% CI = 0.20-0.73, pc = 0.032) and HLA-DRB1*07 (16.34% vs. 26.77%; p = 
      0.003, OR = 0.53, 95% CI = 0.3-0.8, pc = 0.039). A protective association was 
      observed between BL leprosy and HLA-DQB1*02 (18.18% vs. 39.53%; p = 0.005, OR = 
      0.34, 95% CI = 0.15-0.75, pc = 0.025). In reactional patients, a significant 
      association was observed between HLA-B*15 (28.72% vs. 12.76%; p = 0.011, OR = 
      2.75, 95% CI = 1.30-5.85, pc = 0.352) and predisposition to reversal reaction. 
      Haplotype analysis showed that A*02-B*07-C*07-DRB1*15-DQB1*06 (2.97% vs. 1.04%; p 
      = 0.015) and A*02-B*40-C*03-DRB1*13-DQB1*06 (1.73% vs. 0.10%; p = 0.0011) were 
      associated with susceptibility to the B form. The presence of the HLA-DRB1*02 or 
      HLA-DRB1*03/HLA-DQB1*01 haplotypes in B patients (22.05% vs. 33.0%; p = 0.005) 
      suggested the involvement of these haplotypes in this clinical form of the 
      disease. CONCLUSIONS: The results indicate the involvement of HLA class I and 
      class II molecules in B leprosy and reversal reactions; it also suggest a role 
      for HLA in polarization of the disease in this group of patients.
FAU - de Souza-Santana, Fabiana Covolo
AU  - de Souza-Santana FC
AD  - Immunogenetics Laboratory, Instituto Lauro de Souza Lima, Rod. Cte Joao Ribeiro 
      de Barros, km 225/26, Bauru, SP, CEP: 17039-800, Brazil. fsouza@ilsl.br.
FAU - Marcos, Elaine Valim Camarinha
AU  - Marcos EV
AD  - Immunogenetics Laboratory, Instituto Lauro de Souza Lima, Rod. Cte Joao Ribeiro 
      de Barros, km 225/26, Bauru, SP, CEP: 17039-800, Brazil. emarcos@ilsl.br.
FAU - Nogueira, Maria Esther Salles
AU  - Nogueira ME
AD  - Immunology Laboratory, Instituto Lauro de Souza Lima, Rod. Cte Joao Ribeiro de 
      Barros, km 225/26, Bauru, SP, CEP: 17039-800, Brazil. enogueira@ilsl.br.
FAU - Ura, Somei
AU  - Ura S
AD  - Department of Education and Research, Instituto Lauro de Souza Lima, Rod. Cte 
      Joao Ribeiro de Barros, km 225/26, Bauru, SP, CEP: 17039-800, Brazil. 
      sura@ilsl.br.
FAU - Tomimori, Jane
AU  - Tomimori J
AD  - Department of Dermatology, Federal University of Sao Paulo, UNIFESP, Av. Borges 
      Lagoa, 598, Sao Paulo, SP, CEP: 04038-000, Brazil. jane.tomimori@unifesp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150121
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - Brazil
MH  - Case-Control Studies
MH  - Child
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - Humans
MH  - Leprosy, Borderline/*genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium leprae/immunology
MH  - Polymerase Chain Reaction
MH  - Young Adult
PMC - PMC4307149
EDAT- 2015/01/22 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/01/21
CRDT- 2015/01/22 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2015/01/12 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/01/21 00:00 [pmc-release]
AID - s12879-015-0751-0 [pii]
AID - 751 [pii]
AID - 10.1186/s12879-015-0751-0 [doi]
PST - epublish
SO  - BMC Infect Dis. 2015 Jan 21;15:22. doi: 10.1186/s12879-015-0751-0.