PMID- 25605587
OWN - NLM
STAT- MEDLINE
DCOM- 20150811
LR  - 20190109
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 180
IP  - 3
DP  - 2015 Jun
TI  - Indolamine 2,3-dioxygenase expression by monocytes and dendritic cell populations 
      in hepatitis C patients.
PG  - 484-98
LID - 10.1111/cei.12586 [doi]
AB  - Dendritic cells (DCs) play an important role in the induction of the primary 
      immune response to infection. DCs may express the tryptophan-catabolizing enzyme 
      indolamine2,3-dioxygenase (IDO), which is an inducer of immune tolerance. Because 
      there is evidence that chronic hepatitis C virus (HCV) infection leads to 
      functional impairment of certain DC populations, we analysed IDO expression in 
      DCs and monocytes from chronically infected and recovered HCV patients. The IDO1 
      and -2 expression was increased significantly in the monocytes of chronic HCV 
      patients but, interestingly, not in those from recovered patients. The myeloid 
      DCs from chronically infected HCV patients also showed enhanced IDO1 expression, 
      while no change in either IDO1 or -2 was found for plasmacytoid DCs. 
      Up-regulation of IDO1 gene expression was confirmed by the presence of enhanced 
      kynurenine/tryptophan ratios in the plasma from chronic HCV patients. Increased 
      IDO1 and -2 expression was also observed in monocytes from healthy donors 
      infected with an adapted mutant of the HCV JFH-1 strain ex vivo, confirming a 
      direct effect of HCV infection. These changes in IDO expression could be 
      prevented by treatment with the IDO inhibitor 1-methyl tryptophan (1-mT). 
      Furthermore, maturation of monocyte-derived DCs from chronically infected HCV 
      patients, as well as well as monocyte-derived DCs infected ex vivo with HCV, was 
      impaired, but this was reversed by 1-mT treatment. This suggests that IDO 
      inhibitors may be used to treat chronic HCV patients in vivo, in conjunction with 
      current therapies, or to activate DCs from patients ex vivo, such that they can 
      be administered back as a DC-based therapeutic vaccine.
CI  - (c) 2015 British Society for Immunology.
FAU - Schulz, S
AU  - Schulz S
AD  - VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
FAU - Landi, A
AU  - Landi A
AD  - Li Ka Shing Institute of Virology, Medical Microbiology and Immunology, 
      University of Alberta, Edmonton, Alberta, Canada.
FAU - Garg, R
AU  - Garg R
AD  - VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
FAU - Wilson, J A
AU  - Wilson JA
AD  - Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, 
      Canada.
FAU - van Drunen Littel-van den Hurk, S
AU  - van Drunen Littel-van den Hurk S
AD  - VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
AD  - Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, 
      Canada.
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 343-65-7 (Kynurenine)
RN  - 8DUH1N11BX (Tryptophan)
RN  - XD0FY1J13B (1-methyltryptophan)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Chemokines/blood
MH  - Cytokines/blood
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Female
MH  - *Gene Expression
MH  - Gene Expression Regulation/drug effects
MH  - Genotype
MH  - Hepacivirus/genetics/*immunology
MH  - Hepatitis C/drug therapy/*genetics/*immunology/metabolism/virology
MH  - Humans
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/*genetics/metabolism
MH  - Inflammation Mediators/blood
MH  - Kynurenine/blood
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology/*metabolism/virology
MH  - RNA, Messenger/genetics
MH  - Tryptophan/analogs & derivatives/blood/pharmacology/therapeutic use
MH  - Viral Load
MH  - Virus Replication
MH  - Young Adult
PMC - PMC4449777
OTO - NOTNLM
OT  - IDO
OT  - dendritic cell
OT  - hepatitis C virus
OT  - monocyte
EDAT- 2015/01/22 06:00
MHDA- 2015/08/12 06:00
PMCR- 2016/06/01
CRDT- 2015/01/22 06:00
PHST- 2014/07/09 00:00 [received]
PHST- 2014/12/31 00:00 [revised]
PHST- 2015/01/09 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/08/12 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - 10.1111/cei.12586 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2015 Jun;180(3):484-98. doi: 10.1111/cei.12586.