PMID- 25605999
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150121
LR  - 20201001
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 19
IP  - 1
DP  - 2015 Jan
TI  - Airway Smooth Muscle Sensitivity to Methacholine in Precision-Cut Lung Slices 
      (PCLS) from Ovalbumin-induced Asthmatic Mice.
PG  - 65-71
LID - 10.4196/kjpp.2015.19.1.65 [doi]
AB  - Asthma is a chronic inflammatory disease characterized by airway 
      hyperresponsiveness (AHR) and reversible airway obstruction. Methacholine (MCh) 
      is widely used in broncho-provocation test to evaluate airway resistance. For 
      experimental investigation, ovalbumin-induced sensitization is frequently used in 
      rodents (Ova-asthma). However, albeit the inflammatory histology and AHR in vivo, 
      it remains unclear whether the MCh sensitivity of airway smooth muscle isolated 
      from Ova-asthma is persistently changed. In this study, the contractions of 
      airways in precision-cut lung slices (PCLS) from control, Ova-asthma, and IL-13 
      overexpressed transgenic mice (IL-13TG) were compared by analyzing the airway 
      lumen space (AW). The airway resistance in vivo was measured using 
      plethysmograph. AHR and increased inflammatory cells in BAL fluid were confirmed 
      in Ova-asthma and IL-13TG mice. In the PCLS from all three groups, MCh 
      concentration-dependent narrowing of airway lumen (DeltaAW) was observed. In contrast 
      to the AHR in vivo, the EC50 of MCh for DeltaAW from Ova-asthma and IL-13TG were not 
      different from control, indicating unchanged sensitivity to MCh. Although the AW 
      recovery upon MCh-washout showed sluggish tendency in Ova-asthma, the change was 
      also statistically insignificant. Membrane depolarization-induced DeltaAW by 60 mM 
      K(+) (60K-contraction) was larger in IL-13TG than control, whereas 
      60K-contraction of Ova-asthma was unaffected. Furthermore, serotonin-induced DeltaAW 
      of Ova-asthma was smaller than control and IL-13TG. Taken together, the AHR in 
      Ova-asthma and IL-13TG are not reflected in the contractility of isolated airways 
      from PCLS. The AHR of the model animals seems to require intrinsic agonists or 
      inflammatory microenvironment that is washable during tissue preparation.
FAU - Kim, Hae Jin
AU  - Kim HJ
AD  - Department of Physiology, Seoul National University College of Medicine, Seoul 
      110-799, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University 
      College of Medicine, Seoul 110-799, Korea.
FAU - Kim, Yeryung
AU  - Kim Y
AD  - Department of Physiology, Seoul National University College of Medicine, Seoul 
      110-799, Korea.
FAU - Park, Su Jung
AU  - Park SJ
AD  - Department of Physiology, Seoul National University College of Medicine, Seoul 
      110-799, Korea.
FAU - Bae, Boram
AU  - Bae B
AD  - Department of Internal Medicine, Seoul National University College of Medicine, 
      Seoul 110-799, Korea.
FAU - Kang, Hye-Ryun
AU  - Kang HR
AD  - Department of Internal Medicine, Seoul National University College of Medicine, 
      Seoul 110-799, Korea.
FAU - Cho, Sang-Heon
AU  - Cho SH
AD  - Department of Internal Medicine, Seoul National University College of Medicine, 
      Seoul 110-799, Korea.
FAU - Yoo, Hae Young
AU  - Yoo HY
AD  - Chung-Ang University Red Cross College of Nursing, Seoul 100-031, Korea.
FAU - Nam, Joo Hyun
AU  - Nam JH
AD  - hannelopathy Research Center (CRC), Dongguk University College of Medicine, 
      Goyang 410-773, Korea.
FAU - Kim, Woo Kyung
AU  - Kim WK
AD  - hannelopathy Research Center (CRC), Dongguk University College of Medicine, 
      Goyang 410-773, Korea.
FAU - Kim, Sung Joon
AU  - Kim SJ
AD  - Department of Physiology, Seoul National University College of Medicine, Seoul 
      110-799, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University 
      College of Medicine, Seoul 110-799, Korea. ; hannelopathy Research Center (CRC), 
      Dongguk University College of Medicine, Goyang 410-773, Korea.
LA  - eng
PT  - Journal Article
DEP - 20141231
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC4297764
OTO - NOTNLM
OT  - Airway
OT  - Asthma
OT  - Lung slice
OT  - Smooth muscle
EDAT- 2015/01/22 06:00
MHDA- 2015/01/22 06:01
PMCR- 2015/01/01
CRDT- 2015/01/22 06:00
PHST- 2014/10/27 00:00 [received]
PHST- 2014/11/20 00:00 [revised]
PHST- 2014/11/21 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/01/22 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2015.19.1.65 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2015 Jan;19(1):65-71. doi: 10.4196/kjpp.2015.19.1.65. 
      Epub 2014 Dec 31.