PMID- 25606396
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150121
LR  - 20220311
IS  - 2214-5400 (Print)
IS  - 2214-5400 (Electronic)
IS  - 2214-5400 (Linking)
VI  - 2
DP  - 2014 Dec
TI  - Association of interleukin 1 beta (IL-1beta) polymorphism with mRNA expression and 
      risk of non small cell lung cancer.
PG  - 123-33
LID - 10.1016/j.mgene.2013.12.002 [doi]
AB  - INTRODUCTION: Interleukin 1 beta (IL- 1beta), a key proinflammatory cytokine encoded 
      by the interleukin 1 beta gene, has been associated with chronic inflammation and 
      plays an important role in lung inflammatory diseases including lung cancer. 
      Elevated levels of Interleukin 1proteins, in particular interleukin 1 beta 
      greatly enhance the intensity of the inflammatory response. AIM: To study the 
      role of interleukin 1 beta-31C > T and -511 T > C polymorphism in the 
      pathogenesis of non small cell lung cancer (NSCLC). MATERIALS AND METHODS: One 
      hundred and ninety non small cell lung cancer patients and 200 healthy age, sex, 
      smoking and dwelling matched controls were used for polymorphic analysis by 
      polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 
      followed by sequencing. Normal tissues of 48 histopathologically confirmed non 
      small cell lung cancer patients were taken for mRNA expression analysis. 
      Quantitation of interleukin 1 beta was carried out by quantitative real time PCR. 
      RESULT: The T/T genotype of interleukin 1 beta-31 gene was significantly 
      associated with increased risk of NSCLC [(P = 0.001, OR - 2.8 (95%CI 1.52-5.26)]. 
      The interleukin 1 beta - 511 T > C does not show any difference between the NSCLC 
      and control group (P = 0.3, OR - 0.72 (95%CI 0.41-1.28). Quantitative analysis of 
      mRNA showed significant association with interleukin 1 beta T allele as compared 
      to the interleukin 1 beta-31C allele (P = 0.006). CONCLUSION: We conclude that 
      lung cancer risk genotype interleukin 1 beta-31TT results in increased expression 
      of interleukin 1 beta mRNA in lung cancer patients. Our data suggest that this 
      genotype (IL1beta -31TT) in the interleukin 1 beta regulatory region provide a 
      microenvironment with elevated inflammatory stimuli and thus increasing the risk 
      for lung cancer.
FAU - Bhat, Imtiyaz A
AU  - Bhat IA
AD  - Department of Immunology & Molecular Medicine, Sher-i-Kashmir Institute of 
      Medical Sciences, Srinagar, India.
FAU - Naykoo, Niyaz A
AU  - Naykoo NA
AD  - Department of Animal Biotechnology, Sher-i-Kashmir University of Agricultural 
      Sciences, Srinagar, India.
FAU - Qasim, Iqbal
AU  - Qasim I
AD  - Department of Immunology & Molecular Medicine, Sher-i-Kashmir Institute of 
      Medical Sciences, Srinagar, India.
FAU - Ganie, Farooq A
AU  - Ganie FA
AD  - Department of Cardio Vascular and Thoracic Surgery, Sher-i-Kashmir Institute of 
      Medical Sciences, Srinagar India.
FAU - Yousuf, Qaiser
AU  - Yousuf Q
AD  - Advanced Center for Human Genetics, Sher-i-Kashmir Institute of Medical Sciences 
      Srinagar, India.
FAU - Bhat, Bashir A
AU  - Bhat BA
AD  - Department of Plastic Surgery, Sher-i-Kashmir Institute of Medical Sciences 
      Srinagar India.
FAU - Rasool, Roohi
AU  - Rasool R
AD  - Department of Immunology & Molecular Medicine, Sher-i-Kashmir Institute of 
      Medical Sciences, Srinagar, India.
FAU - Aziz, S A
AU  - Aziz SA
AD  - Department of Medical Oncology, Sher-i-Kashmir Institute of Medical Sciences 
      Srinagar India.
FAU - Shah, Zafar Amin
AU  - Shah ZA
AD  - Department of Immunology & Molecular Medicine, Sher-i-Kashmir Institute of 
      Medical Sciences, Srinagar, India.
LA  - eng
PT  - Journal Article
DEP - 20140117
PL  - Netherlands
TA  - Meta Gene
JT  - Meta gene
JID - 101627670
PMC - PMC4287803
OTO - NOTNLM
OT  - Inflammation
OT  - Non small cell lung cancer
OT  - Polymorphism
OT  - Restriction fragment length polymorphism (RFLP)
EDAT- 2015/01/22 06:00
MHDA- 2015/01/22 06:01
PMCR- 2014/01/17
CRDT- 2015/01/22 06:00
PHST- 2013/08/16 00:00 [received]
PHST- 2013/12/09 00:00 [revised]
PHST- 2013/12/09 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2015/01/22 06:01 [medline]
PHST- 2014/01/17 00:00 [pmc-release]
AID - S2214-5400(13)00033-9 [pii]
AID - 10.1016/j.mgene.2013.12.002 [doi]
PST - epublish
SO  - Meta Gene. 2014 Jan 17;2:123-33. doi: 10.1016/j.mgene.2013.12.002. eCollection 
      2014 Dec.