PMID- 25606969
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20150224
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 11
IP  - 5
DP  - 2015 May
TI  - Inhibitory effect of arazyme on the development of atopic dermatitis-like lesions 
      in BALB/c and Nc/Nga mice.
PG  - 3995-4001
LID - 10.3892/mmr.2015.3225 [doi]
AB  - Arazyme is a metalloprotease released by Aranicola proteolyticus that was shown 
      to inhibit cytokine release in HaCaT and endothelial cells. However, the 
      regulatory effects of arazyme in atopic dermatitis remain to be fully understood. 
      In the present study, the anti‑inflammatory effects of arazyme in BALB/c and 
      Nc/Nga mice induced with 2,4‑dinitrochlrobenzene (DNCB) were investigated. BALB/c 
      mice were sensitized with DNCB and were subsequently administered arazyme for 4 
      weeks either orally, dorsally or orally/dorsally. Arazyme administration 
      significantly reduced epidermal thickening and infiltration of inflammatory cells 
      into the dermis compared with the DNCB group. However, serum immunoglobulin E 
      (IgE) levels were not altered by arazyme treatment. Additionally, the level of 
      secretion of interleukins (IL)‑4, ‑5 and ‑13 in the splenocytes of BALB/c mice 
      was elevated following stimulation with concanavalin A, while the increase of 
      IL‑4 and IL‑13 was inhibited by arazyme. Administration of arazyme (25 mg/kg in 
      phosphate‑buffered saline) to Nc/Nga mice that had been sensitized with DNCB for 
      6 weeks reduced the skin severity score compared with that in the DNCB group and 
      inhibited the histological manifestations of atopic dermatitis‑like skin lesions. 
      In addition, the serum IgE levels were reduced in the arazyme‑treated NC/Nga mice 
      relative to the DNCB group. Collectively, these results indicated that arazyme 
      attenuates the development of atopic dermatitis‑like lesions via lowering the 
      levels of IgE and inflammatory cytokines. The results of the present study will 
      aid in the development of effective therapeutic strategies for the treatment of 
      allergic diseases, including atopic dermatitis.
FAU - Kim, In Sik
AU  - Kim IS
AD  - Department of Biomedical Laboratory Science, School of Medicine, Eulji 
      University, Daejeon 301‑746, Republic of Korea.
FAU - Lee, Na Rae
AU  - Lee NR
AD  - Department of Biomedical Laboratory Science, School of Medicine, Eulji 
      University, Daejeon 301‑746, Republic of Korea.
FAU - Baek, Seung Yeop
AU  - Baek SY
AD  - Department of Biomedical Laboratory Science, School of Medicine, Eulji 
      University, Daejeon 301‑746, Republic of Korea.
FAU - Kim, Eun Jeong
AU  - Kim EJ
AD  - Department of Biomedical Laboratory Science, School of Medicine, Eulji 
      University, Daejeon 301‑746, Republic of Korea.
FAU - Kim, Jung Seok
AU  - Kim JS
AD  - Department of Biomedical Laboratory Science, School of Medicine, Eulji 
      University, Daejeon 301‑746, Republic of Korea.
FAU - Jeong, Tae-Sook
AU  - Jeong TS
AD  - Industrial Bio‑material Research Center, Korea Research Institute of Bioscience 
      and Biotechnology, Daejeon 305‑806, Republic of Korea.
FAU - Shin, Dong-Ha
AU  - Shin DH
AD  - Insect Biotech Co., Ltd., Daejeon 305‑811, Republic of Korea.
FAU - Park, Ho-Yong
AU  - Park HY
AD  - Industrial Bio‑material Research Center, Korea Research Institute of Bioscience 
      and Biotechnology, Daejeon 305‑806, Republic of Korea.
FAU - Lee, Ji-Sook
AU  - Lee JS
AD  - Department of Clinical Laboratory Science, Wonkwang Health Science University, 
      Iksan 570‑750, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150119
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.- (Metalloproteases)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/*administration & dosage
MH  - Cytokines/metabolism
MH  - Dermatitis, Atopic/diagnosis/drug therapy/*immunology/*pathology
MH  - Disease Models, Animal
MH  - Immunoglobulin E/blood/immunology
MH  - Metalloproteases/*administration & dosage
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Severity of Illness Index
MH  - Spleen/immunology/metabolism
EDAT- 2015/01/22 06:00
MHDA- 2016/03/25 06:00
CRDT- 2015/01/22 06:00
PHST- 2014/06/23 00:00 [received]
PHST- 2014/12/19 00:00 [accepted]
PHST- 2015/01/22 06:00 [entrez]
PHST- 2015/01/22 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
AID - 10.3892/mmr.2015.3225 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 May;11(5):3995-4001. doi: 10.3892/mmr.2015.3225. Epub 2015 Jan 
      19.