PMID- 25609555
OWN - NLM
STAT- MEDLINE
DCOM- 20160114
LR  - 20191210
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 167
IP  - 3
DP  - 2015 Mar
TI  - The Arabidopsis transcription factor BRASSINOSTEROID INSENSITIVE1-ETHYL 
      METHANESULFONATE-SUPPRESSOR1 is a direct substrate of MITOGEN-ACTIVATED PROTEIN 
      KINASE6 and regulates immunity.
PG  - 1076-86
LID - 10.1104/pp.114.250985 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) are recognized by plant pattern 
      recognition receptors to activate PAMP-triggered immunity (PTI). 
      Mitogen-activated protein kinases (MAPKs), as well as other cytoplasmic kinases, 
      integrate upstream immune signals and, in turn, dissect PTI signaling via 
      different substrates to regulate defense responses. However, only a few direct 
      substrates of these signaling kinases have been identified. Here, we show that 
      PAMP perception enhances phosphorylation of BRASSINOSTEROID INSENSITIVE1-ETHYL 
      METHANESULFONATE-SUPPRESSOR1 (BES1), a transcription factor involved in 
      brassinosteroid (BR) signaling pathway, through pathogen-induced MAPKs in 
      Arabidopsis (Arabidopsis thaliana). BES1 interacts with MITOGEN-ACTIVATED PROTEIN 
      KINASE6 (MPK6) and is phosphorylated by MPK6. bes1 loss-of-function mutants 
      display compromised resistance to bacterial pathogen Pseudomonas syringae pv 
      tomato DC3000. BES1 S286A/S137A double mutation (BES1(SSAA)) impairs PAMP-induced 
      phosphorylation and fails to restore bacterial resistance in bes1 mutant, 
      indicating a positive role of BES1 phosphorylation in plant immunity. BES1 is 
      phosphorylated by glycogen synthase kinase3 (GSK3)-like kinase BR-insensitive2 
      (BIN2), a negative regulator of BR signaling. BR perception inhibits BIN2 
      activity, allowing dephosphorylation of BES1 to regulate plant development. 
      However, BES1(SSAA) does not affect BR-mediated plant growth, suggesting 
      differential residue requirements for the modulation of BES1 phosphorylation in 
      PTI and BR signaling. Our study identifies BES1 as a unique direct substrate of 
      MPK6 in PTI signaling. This finding reveals MAPK-mediated BES1 phosphorylation as 
      another BES1 modulation mechanism in plant cell signaling, in addition to 
      GSK3-like kinase-mediated BES1 phosphorylation and F box protein-mediated BES1 
      degradation.
CI  - (c) 2015 American Society of Plant Biologists. All Rights Reserved.
FAU - Kang, Sining
AU  - Kang S
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.).
FAU - Yang, Fan
AU  - Yang F
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.).
FAU - Li, Lin
AU  - Li L
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.).
FAU - Chen, Huamin
AU  - Chen H
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.).
FAU - Chen, She
AU  - Chen S
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.).
FAU - Zhang, Jie
AU  - Zhang J
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China (S.K., F.Y., J.Z.);University of 
      Chinese Academy of Sciences, Beijing 100049, China (F.Y.); National Institute of 
      Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China (L.L., 
      S.C.); andState Key Laboratory for Biology of Plant Diseases and Insect Pests, 
      Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China (H.C.) zhangjie@im.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150121
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (BES1 protein, Arabidopsis)
RN  - 0 (Brassinosteroids)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 12777-81-0 (Flagellin)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.11.24 (MPK6 protein, Arabidopsis)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Arabidopsis/*enzymology/*immunology/microbiology
MH  - Arabidopsis Proteins/*metabolism
MH  - Brassinosteroids/pharmacology
MH  - DNA-Binding Proteins
MH  - Disease Resistance/immunology
MH  - Enzyme Activation/drug effects
MH  - Flagellin/metabolism
MH  - Hypocotyl/drug effects/growth & development
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Phosphorylation/drug effects
MH  - Phosphoserine/metabolism
MH  - Plant Diseases/immunology/microbiology
MH  - *Plant Immunity/drug effects
MH  - Plant Roots/drug effects/growth & development
MH  - Protein Binding/drug effects
MH  - Pseudomonas syringae/drug effects
MH  - Substrate Specificity/drug effects
PMC - PMC4348755
EDAT- 2015/01/23 06:00
MHDA- 2016/01/15 06:00
PMCR- 2016/03/01
CRDT- 2015/01/23 06:00
PHST- 2015/01/23 06:00 [entrez]
PHST- 2015/01/23 06:00 [pubmed]
PHST- 2016/01/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - pp.114.250985 [pii]
AID - 250985 [pii]
AID - 10.1104/pp.114.250985 [doi]
PST - ppublish
SO  - Plant Physiol. 2015 Mar;167(3):1076-86. doi: 10.1104/pp.114.250985. Epub 2015 Jan 
      21.