PMID- 25612189
OWN - NLM
STAT- MEDLINE
DCOM- 20151019
LR  - 20181202
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 49
IP  - 4
DP  - 2015 Feb 17
TI  - Endosulfan isomers and sulfate metabolite induced reproductive toxicity in 
      Caenorhabditis elegans involves genotoxic response genes.
PG  - 2460-8
LID - 10.1021/es504837z [doi]
AB  - Endosulfan is enlisted as one of the persistent organic pollutants (POPs) and 
      exists in the form of its alpha and beta isomers in the environment as well as in the 
      form of endosulfan sulfate, a toxic metabolite. General endosulfan toxicity has 
      been investigated in various organisms, but the effect of the isomers and sulfate 
      metabolites on reproductive function is unclear. This study was aimed at studying 
      the reproductive dysfunction induced by endosulfan isomers and its sulfate 
      metabolite in Caenorhabditis elegans (C. elegans). We also determined a role for 
      the DNA-damage-checkpoint gene hus-1. Compared to beta-endosulfan and its sulfate 
      metabolite, alpha-endosulfan caused a dramatically higher level of germ cell 
      apoptosis, which was regulated by DNA damage signal pathway. Both endosulfan 
      isomers and the sulfate metabolite induced germ cell cycle arrest. 
      Loss-of-function studies using hus-1, egl-1, and cep-1 mutants revealed that 
      hus-1 specifically influenced the fecundity, hatchability, and sexual ratio after 
      endosulfan exposure. Our data provide clear evidence that the DNA-checkpoint gene 
      hus-1 has an essential role in endosulfan-induced reproductive dysfunction and 
      that alpha-endosulfan exhibited the highest reproductive toxicity among the different 
      forms of endosulfan.
FAU - Du, Hua
AU  - Du H
AD  - Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, 
      CAS and Anhui Province , Hefei, Anhui, PR China.
FAU - Wang, Min
AU  - Wang M
FAU - Dai, Hui
AU  - Dai H
FAU - Hong, Wei
AU  - Hong W
FAU - Wang, Mudi
AU  - Wang M
FAU - Wang, Jingjing
AU  - Wang J
FAU - Weng, Nanyan
AU  - Weng N
FAU - Nie, Yaguang
AU  - Nie Y
FAU - Xu, An
AU  - Xu A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150204
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Ced-4 protein, C elegans)
RN  - 0 (Ced-9 protein, C elegans)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 8QTV9M19B2 (endosulfan sulfate)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (ced-3 protein, C elegans)
RN  - OKA6A6ZD4K (Endosulfan)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caenorhabditis elegans/*drug effects/genetics/*physiology
MH  - Caenorhabditis elegans Proteins/genetics/metabolism
MH  - Calcium-Binding Proteins/genetics
MH  - Caspases/genetics
MH  - DNA Damage/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endosulfan/*analogs & derivatives/chemistry/metabolism/*toxicity
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Isomerism
MH  - Longevity/drug effects
MH  - Male
MH  - Mutation
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Reproduction/drug effects/*genetics
MH  - Sex Ratio
MH  - Signal Transduction/drug effects
MH  - Toxicity Tests/methods
EDAT- 2015/01/23 06:00
MHDA- 2015/10/20 06:00
CRDT- 2015/01/23 06:00
PHST- 2015/01/23 06:00 [entrez]
PHST- 2015/01/23 06:00 [pubmed]
PHST- 2015/10/20 06:00 [medline]
AID - 10.1021/es504837z [doi]
PST - ppublish
SO  - Environ Sci Technol. 2015 Feb 17;49(4):2460-8. doi: 10.1021/es504837z. Epub 2015 
      Feb 4.