PMID- 25612451
OWN - NLM
STAT- MEDLINE
DCOM- 20150312
LR  - 20150123
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 39
IP  - 19
DP  - 2014 Oct
TI  - [Effects of glycyrrhizin acid and licorice flavonoids on LPS-induced cytokines 
      expression in macrophage].
PG  - 3841-5
AB  - Glycyrrhizin acid and licorice flavonoids are the component of Glycyrrhiza 
      uralensis Fisch root that has been used for various medicinal purposes in 
      traditional oriental medicine for thousands of years. Macrophages as a principal 
      component of immune system play an important role in the initiation, modulation 
      and final activation of immune response against pathogens. In the present study, 
      glycyrrhizin acid and licorice flavonoids was investigated the anti-inflammatory 
      effect on lipopolysaccharide (LPS)-induced macrophage cell line of RAW264.7. 
      Well-grown RAW264.7 cells were collected and randomly divided into the blank 
      control group, the LPS(1 mg x L(-1)) group, the dexamethasone (5 mg x L(-1)) with 
      LPS group, the glycyrrhizin acid (400, 80, 16 mg x L(-1)) with LPS group and the 
      licorice flavonoids (200, 40, 8 mg x L(-1)) with LPS group. RAW264.7 cells were 
      cultured in 24-well plates, pre-incubated for 4 h with different concentrations 
      of dexamethasone, glycyrrhizin acid, or licorice flavonoids. Then cells were 
      stimulated for 20 h with LPS. The supernatant of culture medium was collected 
      from each well and determinated the concentrations of cytokines by means of 
      BioPlex mouse cytokines assay. Compared with the control group, the LPS group 
      could significantly induced relatively high levels of granulocyte colony 
      stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor( 
      GM-CSF), macrophage inflammatory protein-1 alpha (MIP-1alpha), macrophage 
      inflammatory protein-1 beta (MIP-1beta), regulated upon activation normal T cell 
      expressed and secreted factor (RANTES), tumor necrosis factor alpha ( TNF-alpha), 
      monocyte chemotactic protein 1 (MCP-1), chemokine (C-X-C motif) ligand 1 (KC), 
      eotaxin, interleukin(IL)-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12 (p40), 
      IL-12 (p70), IL-13, and IL-17 secretion (P < 0.05). The glycyrrhizin acid 
      significantly inhibited IL-1beta, IL-3, IL-5, IL-6, IL-10, IL-12 (p40), IL-12 (p70), 
      IL-13, Eotaxin and TNF-alpha secreted by LPS-stimulated RAW264.7 cells (P < 0.05). 
      The expression levels of IL-6 and Eotaxin were observably decreased in the 
      licorice flavonoids with LPS group (P < 0.05). The data presented here suggested 
      that the glycyrrhizin acid and licorice flavonoids modulate various cytokines 
      secreted by macrophages and were important anti-inflammatory constituent of 
      Licorice.
FAU - Liu, Zhao
AU  - Liu Z
FAU - Zhong, Ju-Ying
AU  - Zhong JY
FAU - Gao, Er-Ning
AU  - Gao EN
FAU - Yang, Hong
AU  - Yang H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Cytokines)
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 6FO62043WK (Glycyrrhizic Acid)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytokines/*genetics/immunology
MH  - Flavonoids/*pharmacology
MH  - Glycyrrhiza/*chemistry
MH  - Glycyrrhizic Acid/*pharmacology
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/*drug effects/immunology
MH  - Mice
EDAT- 2015/01/24 06:00
MHDA- 2015/03/13 06:00
CRDT- 2015/01/24 06:00
PHST- 2015/01/24 06:00 [entrez]
PHST- 2015/01/24 06:00 [pubmed]
PHST- 2015/03/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(19):3841-5.