PMID- 25612555
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20180314
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 90
IP  - 6
DP  - 2016 Nov
TI  - The utility of multiparametric seven-color flow cytometry in the detection of 
      double hit lymphoma in ascitic fluid samples.
PG  - 543-545
LID - 10.1002/cyto.b.21227 [doi]
AB  - Double-hit lymphoma (DHL) is a rare type of lymphoma with concurrent chromosomal 
      translocations of C-MYC with BCL2 or BCL6, associated with unfavorable prognosis. 
      We describe a case of DHL in a 79-year-old female patient previously diagnosed 
      with diffuse large B-cell lymphoma (DLBCL) with an early relapse in the ascitic 
      fluid. A seven-color multiparametric flow cytometry immunophenotyping study of 
      the ascitic fluid was carried out, and revealed 99.78% of large in size and high 
      cellular complexity B-cells positive for CD19, CD10 (64.27%), CD45 dim, CD22 dim, 
      CD25 (60%), CD43 bright, CD38 bright, and IgM (18.53%); and negative for CD20, 
      CD5, CD23, CD79b, CD103, CD200, CD11c, and FMC7, and 78.99% without light chain 
      expression and 21% with Lambda chain restriction. Due to the expression of CD19 
      and CD10 with overexpression of BCL-2 protein and due to CD43 and CD38 positivity 
      detected, those cells showed features between DLBCL and Burkitt lymphoma. 
      Fluorescence in situ hybridization (FISH) confirmed both c-MYC/IGH and BCL2/IGH 
      rearrangement. Our findings may help to identify cases requiring additional 
      cytogenetic analysis. (c) 2015 International Clinical Cytometry Society.
CI  - (c) 2015 International Clinical Cytometry Society.
FAU - Cordoba, Raul
AU  - Cordoba R
AD  - Lymphoma Unit, Hematology Deparment, Fundacion Jimenez Diaz University Hospital, 
      Health Research Institute IIS-FJD, Madrid, Spain.
FAU - Alvarez, Beatriz
AU  - Alvarez B
AD  - Central Laboratory, Flow Cytometry Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Masso, Pilar
AU  - Masso P
AD  - Hematology Department, Infanta Sofia University Hospital, San Sebastian De Los 
      Reyes, Madrid, Spain.
FAU - Gonzalez, Fernando Ataulfo
AU  - Gonzalez FA
AD  - Central Laboratory, Hematology Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Conejo, Luz
AU  - Conejo L
AD  - Central Laboratory, Flow Cytometry Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Velasco, Diego
AU  - Velasco D
AD  - Central Laboratory, Hematology Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Alonso, Juan-Manuel
AU  - Alonso JM
AD  - Central Laboratory, Hematology Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Villarrubia, Jesus
AU  - Villarrubia J
AD  - Central Laboratory, Hematology Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Cava, Fernando
AU  - Cava F
AD  - Central Laboratory, Hematology Unit, Infanta Sofia University Hospital, San 
      Sebastian De Los Reyes, Madrid, Spain.
FAU - Llamas, Pilar
AU  - Llamas P
AD  - Lymphoma Unit, Hematology Deparment, Fundacion Jimenez Diaz University Hospital, 
      Health Research Institute IIS-FJD, Madrid, Spain.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20150602
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
SB  - IM
MH  - Aged
MH  - Ascitic Fluid/*pathology
MH  - Burkitt Lymphoma/diagnosis/pathology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Immunophenotyping/methods
MH  - Lymphoma, Large B-Cell, Diffuse/*diagnosis/*pathology
OTO - NOTNLM
OT  - double-hit
OT  - flow cytometry
OT  - lymphoma
EDAT- 2015/01/24 06:00
MHDA- 2017/10/19 06:00
CRDT- 2015/01/24 06:00
PHST- 2014/11/09 00:00 [received]
PHST- 2015/01/14 00:00 [revised]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2015/01/24 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2015/01/24 06:00 [entrez]
AID - 10.1002/cyto.b.21227 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2016 Nov;90(6):543-545. doi: 10.1002/cyto.b.21227. Epub 
      2015 Jun 2.