PMID- 25613158
OWN - NLM
STAT- MEDLINE
DCOM- 20151013
LR  - 20211103
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 35
IP  - 2
DP  - 2015
TI  - Mesenchymal stem cells mitigate cirrhosis through BMP7.
PG  - 433-40
LID - 10.1159/000369708 [doi]
AB  - BACKGROUND/AIMS: Transplantation of mesenchymal stem cells (MSCs) has therapeutic 
      effects on various diseases, while its effect on developing cirrhosis as well as 
      the underlying mechanism remained largely unknown. METHODS: Twenty C57BL/6 mice 
      were randomly separated into 2 groups of ten each. One group received 
      transplantation of MSCs, while the other group received saline as control. The 
      mice then received intraperitoneal injection of carbon tetrachloride (CCl4) twice 
      per week for 8 weeks to develop cirrhosis. After another 4 weeks, the levels of 
      cirrhosis in these mice were evaluated by liver fibrosis area, portal pressure, 
      sodium balance and excretion. Transcripts of transforming growth factor beta 1 
      (TGFbeta1) and bone morphogenic protein 7 (BMP7) in the mouse livers were quantified 
      by RT-qPCR. BMP7-depleted MSCs were prepared and applied in this model, and 
      compared to MSCs. RESULTS: Liver fibrosis, portal hypertension and sodium 
      retention that were developed by CCl4, were all significantly alleviated by MSCs 
      transplantation, which decreased TGFbeta1 levels and increased BMP7 levels in the 
      injured liver. MSCs were found to express extremely high levels of BMP7. 
      Knockdown of BMP7 in MSCs completely abolished the protective effect of MSCs 
      against CCl4-induced cirrhosis. CONCLUSIONS: MSCs mitigate cirrhosis through 
      their production of BMP7 against the fibrogenic effect of TGFbeta1 in the injured 
      liver.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Li, Bing
AU  - Li B
AD  - Liver Fibrosis Diagnosis and Treatment Center, 302 Hospital of PLA, Beijing, 
      China.
FAU - Shao, Qing
AU  - Shao Q
FAU - Ji, Dong
AU  - Ji D
FAU - Li, Fan
AU  - Li F
FAU - Chen, Guofeng
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150115
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (bmp7 protein, mouse)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 7/antagonists & inhibitors/*genetics
MH  - Carbon Tetrachloride
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Hypertension, Portal/pathology/*therapy
MH  - Liver Cirrhosis, Experimental/chemically induced/genetics/pathology/*therapy
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Random Allocation
MH  - Transforming Growth Factor beta1/*genetics
EDAT- 2015/01/24 06:00
MHDA- 2015/10/16 06:00
CRDT- 2015/01/24 06:00
PHST- 2014/11/27 00:00 [accepted]
PHST- 2015/01/24 06:00 [entrez]
PHST- 2015/01/24 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 000369708 [pii]
AID - 10.1159/000369708 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2015;35(2):433-40. doi: 10.1159/000369708. Epub 2015 Jan 
      15.