PMID- 25614742
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150123
LR  - 20201001
IS  - 1687-8876 (Print)
IS  - 1687-8884 (Electronic)
IS  - 1687-8876 (Linking)
VI  - 2014
DP  - 2014
TI  - Effect of serum and oxygen concentration on gene expression and secretion of 
      paracrine factors by mesenchymal stem cells.
PG  - 601063
LID - 10.1155/2014/601063 [doi]
LID - 601063
AB  - Mesenchymal stem cells (MSC) secrete paracrine factors that may exert a 
      protective effect on the heart after coronary artery occlusion. This study was 
      done to determine the effect of hypoxia and serum levels on the mRNA expression 
      and secretion of paracrine factors. Mouse bone marrow MSC were cultured with 5% 
      or 20% serum and in either normoxic (21% O2) or hypoxic (1% O2) conditions. 
      Expression of mRNA for vascular endothelial growth factor (VEGF), monocyte 
      chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha), 
      MIP-1beta, and matrix metalloproteinase-2 (MMP-2) was determined by RT-qPCR. 
      Secretion into the culture media was determined by ELISA. Hypoxia caused a 
      reduction in gene expression for MCP-1 and an increase for VEGF (5% serum), 
      MIP-1alpha, MIP-1beta, and MMP-2. Serum reduction lowered gene expression for VEGF 
      (normoxia), MCP-1 (hypoxia), MIP-1alpha (hypoxia), MIP-1beta (hypoxia), and MMP-2 
      (hypoxia) and increased gene expression for MMP-2 (normoxia). The level of 
      secretion of these factors into the media generally paralleled gene expression 
      with some exceptions. These data demonstrate that serum and oxygen levels have a 
      significant effect on the gene expression and secretion of paracrine factors by 
      MSC which will affect how MSC interact in vivo during myocardial ischemia.
FAU - Page, Patrick
AU  - Page P
AUID- ORCID: 0000-0001-5805-2063
AD  - Department of Biology, Trinity Christian College, 6601 W. College Drive, Palos 
      Heights, IL 60463, USA.
FAU - DeJong, Joshua
AU  - DeJong J
AUID- ORCID: 0000-0002-9911-917X
AD  - Department of Biology, Trinity Christian College, 6601 W. College Drive, Palos 
      Heights, IL 60463, USA.
FAU - Bandstra, Alaina
AU  - Bandstra A
AUID- ORCID: 0000-0003-3274-7072
AD  - Illinois College of Optometry, 3241 S. Michigan Avenue, Chicago, IL 60616, USA.
FAU - Boomsma, Robert A
AU  - Boomsma RA
AD  - Department of Biology, Trinity Christian College, 6601 W. College Drive, Palos 
      Heights, IL 60463, USA.
LA  - eng
PT  - Journal Article
DEP - 20141229
PL  - United States
TA  - Int J Cell Biol
JT  - International journal of cell biology
JID - 101517861
PMC - PMC4295344
EDAT- 2015/01/24 06:00
MHDA- 2015/01/24 06:01
PMCR- 2014/12/29
CRDT- 2015/01/24 06:00
PHST- 2014/07/22 00:00 [received]
PHST- 2014/10/31 00:00 [revised]
PHST- 2014/12/05 00:00 [accepted]
PHST- 2015/01/24 06:00 [entrez]
PHST- 2015/01/24 06:00 [pubmed]
PHST- 2015/01/24 06:01 [medline]
PHST- 2014/12/29 00:00 [pmc-release]
AID - 10.1155/2014/601063 [doi]
PST - ppublish
SO  - Int J Cell Biol. 2014;2014:601063. doi: 10.1155/2014/601063. Epub 2014 Dec 29.